BMRB Entry 51192

Title:
Hydrogen exchange rates of human DHFR
Deposition date:
2021-11-24
Original release date:
2022-05-18
Authors:
Wagnkilde, Morten; Teilum, Kaare
Citation:

Citation: Kampmeyer, Caroline; Larsen-Ledet, Sven; Wagnkilde, Morten; Michelsen, Mathias; Iversen, Henriette; Nielsen, Sofie; Lindemose, Soren; Caregnato, Alberto; Ravid, Tommer; Stein, Amelie; Teilum, Kaare; Lindorff-Larsen, Kresten; Hartmann-Petersen, Rasmus. "Disease-linked mutations cause exposure of a protein quality control degron"  Structure 30, 1245-1253 (2022).
PubMed: 35700725

Assembly members:

Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_FOL, non-polymer, 441.397 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSUMO_DHFR

Data sets:
Data typeCount
H exchange rates104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein1
2ligand2

Entities:

Entity 1, Protein - Formula weight is not available

1   METVALGLYSERLEUASNCYSILEVALALA
2   VALSERGLNASNMETGLYILEGLYLYSASN
3   GLYASPLEUPROTRPPROPROLEUARGASN
4   GLUPHEARGTYRPHEGLNARGMETTHRTHR
5   THRSERSERVALGLUGLYLYSGLNASNLEU
6   VALILEMETGLYLYSLYSTHRTRPPHESER
7   ILEPROGLULYSASNARGPROLEULYSGLY
8   ARGILEASNLEUVALLEUSERARGGLULEU
9   LYSGLUPROPROGLNGLYALAHISPHELEU
10   SERARGSERLEUASPASPALALEULYSLEU
11   THRGLUGLNPROGLULEUALAASNLYSVAL
12   ASPMETVALTRPILEVALGLYGLYSERSER
13   VALTYRLYSGLUALAMETASNHISPROGLY
14   HISLEULYSLEUPHEVALTHRARGILEMET
15   GLNASPPHEGLUSERASPTHRPHEPHEPRO
16   GLUILEASPLEUGLULYSTYRLYSLEULEU
17   PROGLUTYRPROGLYVALLEUSERASPVAL
18   GLNGLUGLULYSGLYILELYSTYRLYSPHE
19   GLUVALTYRGLULYSASNASP

Entity 2, ligand - C19 H19 N7 O6 - 441.397 Da.

1   FOL

Samples:

sample_1: dihydrofolate reductase, [U-100% 15N], 0.4 mM; folate 425 uM; D2O 55 M; K-phosphate 50 mM; KCl 50 mM; DTT 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe v9.9 - data analysis

NMR spectrometers:

  • Bruker AVANCE III HD 750 MHz

Related Database Links:

UniProt P00374
AlphaFold Q6IRW8