BMRB Entry 51187

Title:
Backbone and sidechain NMR assignments of the J-domain of co-chaperone Sis1 in complex with EEVD peptide of HSP70
Deposition date:
2021-11-23
Original release date:
2022-11-23
Authors:
Matos, Carolina; Pinheiro, Glaucia; Ramos, Carlos; Almeida, Fabio
Citation:

Citation: Matos, Carolina; Pinheiro, Glaucia; Ramos, Carlos; Almeida, Fabio. "Backbone and sidechain NMR assignments of residues 1-81 from yeast Sis1 in complex with an Hsp70 C-terminal EEVD peptide"  Biomol. NMR Assign. 17, 239-242 (2023).
PubMed: 37589820

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
entity_2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt32a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts77
1H chemical shifts548

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1J-domain of Sis11
2HSP70 C-terminal tail octapeptide2

Entities:

Entity 1, J-domain of Sis1 84 residues - Formula weight is not available

1   GLYMETTHRSERVALLYSGLUTHRLYSLEU
2   TYRASPLEULEUGLYVALSERPROSERALA
3   ASNGLUGLNGLULEULYSLYSGLYTYRARG
4   LYSALAALALEULYSTYRHISPROASPLYS
5   PROTHRGLYASPTHRGLULYSPHELYSGLU
6   ILESERGLUALAPHEGLUILELEUASNASP
7   PROGLNLYSARGGLUILETYRASPGLNTYR
8   GLYLEUGLUALAALAARGSERGLYGLYPRO
9   SERPHEGLYPRO

Entity 2, HSP70 C-terminal tail octapeptide 8 residues - Formula weight is not available

1   GLYPROTHRILEGLUGLUVALASP

Samples:

sample_1: Sis1 J-domain, [U-95% 13C; U-95% 15N], 1 mM; GPTIEEVD 4 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

ANALYSIS - chemical shift assignment, chemical shift calculation, data analysis

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Related Database Links:

UNIPROT P11142 P11142
AlphaFold Q9H3R6 Q9H3R6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks