BMRB Entry 51162

Title:
N88R CtRNHI
Deposition date:
2021-10-30
Original release date:
2022-03-28
Authors:
Martin, James; Palmer, Arthur
Citation:

Citation: Martin, James; Palmer, Arthur. "Comparisons of Ribonuclease HI Homologs and Mutants Uncover a Multistate Model for Substrate Recognition"  J. Am. Chem. Soc. 144, 5342-5349 (2022).
PubMed: 35312304

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Clostridium tepidum   Taxonomy ID: 1962263   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium tepidum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-25b(+)

Data sets:
Data typeCount
15N chemical shifts145
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N88R CtRNHI1

Entities:

Entity 1, N88R CtRNHI 146 residues - Formula weight is not available

1   METGLULYSTHRILETHRILETYRTHRASP
2   GLYALAALASERGLYASNPROGLYLYSGLY
3   GLYTRPGLYALALEULEUMETTYRGLYSER
4   SERARGLYSGLUILESERGLYTYRASPPRO
5   ALATHRTHRASNASNARGMETGLULEUMET
6   ALAALAILELYSGLYLEUGLUALALEULYS
7   GLUPROALAARGVALGLNLEUTYRSERASP
8   SERALATYRLEUVALASNALAMETASNGLU
9   GLYTRPLEULYSARGTRPVALLYSARGGLY
10   TRPLYSTHRALAALALYSLYSPROVALGLU
11   ASNILEASPLEUTRPGLNGLUILELEULYS
12   LEUTHRTHRLEUHISARGVALTHRPHEHIS
13   LYSVALLYSGLYHISSERASPASNPROTYR
14   ASNSERARGALAASPGLULEUALAARGLEU
15   ALAILELYSGLUASNSER

Samples:

sample_1: V98A E. coli Ribonuclease HI, [U-100% 13C; U-100% 15N], 325 uM; deuterated sodium acetate 50 mM; sodium chloride 50 mM; sodium azide 0.02 % (w/v); DSS 3 mM

sample_conditions_1: ionic strength: 0.050 M; pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks