BMRB Entry 51147

Title:
Backbone NMR assignments of the human TRPV4 intrinsically disordered N-terminus
Deposition date:
2021-10-19
Original release date:
2022-05-05
Authors:
Goretzki, Benedikt; Tebbe, Frederike; Hellmich, Ute; Mitrovic, Sarah-Ana
Citation:

Citation: Goretzki, Benedikt; Tebbe, Frederike; Mitrovic, Sarah-Ana; Hellmich, Ute. "Backbone NMR assignments of the extensive human and chicken TRPV4 N-terminal intrinsically disordered regions as important players in ion channel regulation"  Biomol. NMR Assignments 16, 205-212 (2022).
PubMed: 35451798

Assembly members:

Assembly members:
entity_1, polymer, 147 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts419
15N chemical shifts124
1H chemical shifts467

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRPV4-IDR1

Entities:

Entity 1, TRPV4-IDR 147 residues - Formula weight is not available

1   ALAASPSERSERGLUGLYPROARGALAGLY
2   PROGLYGLUVALALAGLULEUPROGLYASP
3   GLUSERGLYTHRPROGLYGLYGLUALAPHE
4   PROLEUSERSERLEUALAASNLEUPHEGLU
5   GLYGLUASPGLYSERLEUSERPROSERPRO
6   ALAASPALASERARGPROALAGLYPROGLY
7   ASPGLYARGPROASNLEUARGMETLYSPHE
8   GLNGLYALAPHEARGLYSGLYVALPROASN
9   PROILEASPLEULEUGLUSERTHRLEUTYR
10   GLUSERSERVALVALPROGLYPROLYSLYS
11   ALAPROMETASPSERLEUPHEASPTYRGLY
12   THRTYRARGHISHISSERSERASPASNLYS
13   ARGTRPARGLYSLYSILEILEGLULYSGLN
14   PROGLNSERPROLYSALAPROALAPROGLN
15   PROPROPROILELEULYSVAL

Samples:

sample_1: TRPV4-IDR, [U-100% 13C; U-100% 15N], 200 uM; DSS 100 uM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA v1.9.1.4 - chemical shift assignment

TOPSPIN v4.1 - processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 900 MHz

Related Database Links:

UniProtKB Q9HBA0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks