BMRB Entry 51078

Title:
Backbone and side-chain resonance assignments of the harmonin homology domain 1 (HHD1) of human RTEL1
Deposition date:
2021-09-07
Original release date:
2022-05-06
Authors:
Ghosh, Meenakshi; Kumar, Niranjan; Manikandan, Parthasarathy; Basak, Sanmoyee; Singh, Mahavir
Citation:

Citation: Kumar, Niranjan; Ghosh, Meenakshi; Manikandan, Parthasarathy; Basak, Sanmoyee; Deepa, Akula; Singh, Mahavir. "Resonance assignment and secondary structure of the tandem harmonin homology domains of human RTEL1"  Biomol. NMR Assign. 16, 159-164 (2022).
PubMed: 35320499

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts92
1H chemical shifts498

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RTEL1_HHD11

Entities:

Entity 1, RTEL1_HHD1 104 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERGLUPROVALALAGLYALAGLNTHRASP
3   ARGALALYSLEUPHEMETVALALAVALLYS
4   GLNGLULEUSERGLNALAASNPHEALATHR
5   PHETHRGLNALALEUGLNASPTYRLYSGLY
6   SERASPASPPHEALAALALEUALAALACYS
7   LEUGLYPROLEUPHEALAGLUASPPROLYS
8   LYSHISASNLEULEUGLNGLYPHETYRGLN
9   PHEVALARGPROHISHISLYSGLNGLNPHE
10   GLUGLUVALCYSILEGLNLEUTHRGLYARG
11   GLYCYSGLYTYR

Samples:

sample_1: RTEL1_HHD1, [U-99% 13C; U-99% 15N], 0.7 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

VNMRj - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Agilent DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks