BMRB Entry 51075

Title:
FLN5 filamin domain side-chain chemical shift assignments
Deposition date:
2021-09-06
Original release date:
2021-09-12
Authors:
Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John
Citation:

Citation: Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John. "Nascent chain dynamics and ribosome interactions within folded ribosome-nascent chain complexes observed by NMR spectroscopy"  Chem. Sci. 12, 13120-13126 (2021).
PubMed: 34745542

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Dictyostelium discoideum   Taxonomy ID: 44689   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLDC

Data sets:
Data typeCount
13C chemical shifts258
15N chemical shifts96
1H chemical shifts538

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FLN51

Entities:

Entity 1, FLN5 114 residues - Formula weight is not available

Fifth Domain from filamin. First 9 residues are non-native affinity tag.

1   METHISHISHISHISHISHISALASERLYS
2   PROALAPROSERALAGLUHISSERTYRALA
3   GLUGLYGLUGLYLEUVALLYSVALPHEASP
4   ASNALAPROALAGLUPHETHRILEPHEALA
5   VALASPTHRLYSGLYVALALAARGTHRASP
6   GLYGLYASPPROPHEGLUVALALAILEASN
7   GLYPROASPGLYLEUVALVALASPALALYS
8   VALTHRASPASNASNASPGLYTHRTYRGLY
9   VALVALTYRASPALAPROVALGLUGLYASN
10   TYRASNVALASNVALTHRLEUARGGLYASN
11   PROILELYSASNMETPROILEASPVALLYS
12   CYSILEGLUGLY

Samples:

sample_1: FLN5, [U-13C; U-15N], 500 uM; HEPES 10 mM; ammonium chloride 30 mM; magnesium chloride 12 mM; EDTA 1 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NH-TOCSYsample_1isotropicsample_conditions_1
3D (H)C(CO)NH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.414 - chemical shift assignment

NMRPipe - processing

TOPSPIN - collection

SMILE - processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks