BMRB Entry 51070

Title:
PRC1_DD
Deposition date:
2021-08-26
Original release date:
2022-06-24
Authors:
Tan, Fei
Citation:

Citation: Tan, Fei; Xu, Jin. "Validation of the solution structure of dimerization domain of PRC1"  PLoS One 17, e0270572-e0270572 (2022).
PubMed: 35930764

Assembly members:

Assembly members:
entity_1, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet21A

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts68
1H chemical shifts517

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Prc1 N terminal domain (aa:1-65), chain 11
2Prc1 N terminal domain (aa:1-65), chain 21

Entities:

Entity 1, Prc1 N terminal domain (aa:1-65), chain 1 67 residues - Formula weight is not available

1   METARGARGSERGLUVALLEUALAGLUGLU
2   SERILEVALCYSLEUGLNLYSALALEUASN
3   HISLEUARGGLUILETRPGLULEUILEGLY
4   ILEPROGLUASPGLNARGLEUGLNARGTHR
5   GLUVALVALLYSLYSHISILELYSGLULEU
6   LEUASPMETMETILEALAGLUGLUGLUSER
7   LEULYSGLUARGLEUGLUHIS

Samples:

sample_1: PRC1_DD, [U-100% 13C; U-100% 15N], 0.75 mM; PRC1_DD, [U-95% 15N], 0.75 mM; phosphate 50 mM; NaCl 150 mM; DTT 5 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CANDID - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks