BMRB Entry 51064

Title:
NaKd18 in 600 mM Na+
Deposition date:
2021-08-24
Original release date:
2021-11-01
Authors:
Lewis, Adam; Kurauskas, Vilius; Tonelli, Marco; Henzler-Wildman, Katherine
Citation:

Citation: Lewis, Adam; Kurauskas, Vilius; Tonelli, Marco; Henzler-Wildman, Katherine. "Ion-dependent structure, dynamics, and allosteric coupling in a non-selective cation channel"  Nat. Commun. 12, 6225-6225 (2021).
PubMed: 34711838

Assembly members:

Assembly members:
entity_1, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus cereus   Taxonomy ID: 1396   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus cereus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts83
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NaKd18, monomer 11
2NaKd18, monomer 21
3NaKd18, monomer 31
4NaKd18, monomer 41

Entities:

Entity 1, NaKd18, monomer 1 92 residues - Formula weight is not available

1   TRPLYSASPLYSGLUPHEGLNVALLEUPHE
2   VALLEUTHRILELEUTHRLEUILESERGLY
3   THRILEPHETYRSERTHRVALGLUGLYLEU
4   ARGPROILEASPALALEUTYRPHESERVAL
5   VALTHRLEUTHRTHRVALGLYASPGLYASN
6   PHESERPROGLNTHRASPPHEGLYLYSILE
7   PHETHRILELEUTYRILEPHEILEGLYILE
8   GLYLEUVALPHEGLYPHEILEHISLYSLEU
9   ALAVALASNVALGLNLEUPROSERILELEU
10   SERASN

Samples:

sample_1: NaKd18, [U-100% 13C; U-100% 15N; U-80% 2H], 550 uM; D2O 7%; MOPS 100 mM; NaCl 600 mM; DSS 50 uM

sample_2: NaKd18, [U-15N; U-2H], 550 uM; D2O 7%; MOPS 100 mM; NaCl 600 mM

sample_3: NaKd18, [U-15N; U-2H, ILV 13C, 1H], 550 uM; MOPS 100 mM; NaCl 600 mM; DSS 50 uM

sample_conditions_1: ionic strength: 600 mM; pH: 7; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HMQCsample_3isotropicsample_conditions_1
3D 13C-separated NOESYsample_3isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 750 MHz
  • Bruker AVANCE III 600 MHz
  • Varian VNMRS 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks