BMRB Entry 51061

Name: mMLKL
Published: 2022-03-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51061

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

mMLKL

Deposition date:

2021-08-19

Original release date:

2022-03-08

Authors:

Sethi, Ashish; Horne, Chris; Gooley, Paul R.; Murphy, James

Citation:

Citation: Sethi, Ashish; Horne, Christopher; Fitzgibbon, Cheree; Wilde, Karyn; Davies, Katherine; Garnish, Sarah; Jacobsen, Annette; Samson, Andre; Hildebrand, Joanne; Wardak, Ahmad; Czabotar, Peter; Petrie, Emma; Gooley, Paul; Murphy, James. "Membrane permeabilization is mediated by distinct epitopes in mouse and human orthologs of the necroptosis effector, MLKL" Cell Death Diff. 29, 1804-1815 (2022).
PubMed: 35264780

Assembly members:

Assembly members:
entity_1, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETNusH Htb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGSMDKLGQIIKLGQLIY EQCEKMKYCRKQCQRLGNRV HGLLQPLQRLQAQGKKNLPD DITAALGRFDEVLKEANQQI EKFSKKSHIWKFVSVGNDKI LFHEVNEKLRDVWEELLLLL QVYHWNTVSDVSQPASWQQE DRQDAEEDGNENMKVILMQL QIS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	244
15N chemical shifts	151
1H chemical shifts	151

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mMLKL monomer	1

Entities:

Entity 1, mMLKL monomer 163 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

SER

MET

ASP

LYS

LEU

GLY

2

GLN

ILE

LYS

LEU

GLY

GLN

LEU

ILE

TYR

3

GLU

GLN

CYS

GLU

LYS

MET

LYS

TYR

CYS

ARG

4

LYS

GLN

CYS

GLN

ARG

LEU

GLY

ASN

ARG

VAL

5

HIS

GLY

LEU

GLN

PRO

LEU

GLN

ARG

LEU

6

GLN

ALA

GLN

GLY

LYS

ASN

LEU

PRO

ASP

7

ASP

ILE

THR

ALA

LEU

GLY

ARG

PHE

ASP

8

GLU

VAL

LEU

LYS

GLU

ALA

ASN

GLN

ILE

9

GLU

LYS

PHE

SER

LYS

SER

HIS

ILE

TRP

10

LYS

PHE

VAL

SER

VAL

GLY

ASN

ASP

LYS

ILE

11

LEU

PHE

HIS

GLU

VAL

ASN

GLU

LYS

LEU

ARG

12

ASP

VAL

TRP

GLU

LEU

13

GLN

VAL

TYR

HIS

TRP

ASN

THR

VAL

SER

ASP

14

VAL

SER

GLN

PRO

ALA

SER

TRP

GLN

GLU

15

ASP

ARG

GLN

ASP

ALA

GLU

ASP

GLY

ASN

16

GLU

ASN

MET

LYS

VAL

ILE

LEU

MET

GLN

LEU

17

GLN

ILE

SER

Samples:

sample_1: mMLKL(1-158), [U-13C; U-15N; U-2H], 352 uM; HEPES 20 mM; sodium chloride 100 mM; TCEP 1 mM; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks