BMRB Entry 5103

Title:
1H, 13C and 15N resonance assignments of the calcium binding protein S100P
Deposition date:
2001-08-10
Original release date:
2015-09-02
Authors:
Volk, David; Thiviyanathan, Varatharasa; Gorenstein, David; Luxon, Bruce
Citation:

Citation: Lee, Y.; Volk, David; Thiviyanathan, Varatharasa; Kleerekoper, Q.; Gribenko, A.; Zhang, S.; Gorenstein, David; Makhatadze, G.; Luxon, Bruce. "NMR structure of the Apo-S100P protein"  J. Biomol. NMR 29, 399-402 (2004).
PubMed: 15213440

Assembly members:

Assembly members:
human s100p, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts324
15N chemical shifts91
1H chemical shifts542

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1s100p subunit 11
2s100p subunit 21

Entities:

Entity 1, s100p subunit 1 95 residues - Formula weight is not available

1   METTHRGLULEUGLUALAALAMETGLYMET
2   ILEILEASPVALPHESERARGTYRSERGLY
3   SERGLUGLYSERTHRGLNTHRLEUTHRLYS
4   GLYGLULEULYSVALLEUMETGLULYSGLU
5   LEUPROGLYPHELEUGLNSERGLYLYSASP
6   LYSASPALAVALASPLYSLEULEULYSASP
7   LEUASPALAASNGLYASPALAGLNVALASP
8   PHESERGLUPHEILEVALPHEVALALAALA
9   ILETHRSERALASERHISLYSTYRPHEGLU
10   LYSTHRGLYLEULYS

Samples:

sample_1: human s100p, [U-13C; U-15N], 2.3 – 3.0 mM

Condition_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HCACOCANHnot availablenot availablenot available
HNCOnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available

Software:

FELIX v97 - peak assignments, peak picking

NMR spectrometers:

  • Varian UnityPlus 600 MHz
  • Varian UnityPlus 750 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks