BMRB Entry 50963

Title:
Backbone Resonance Assignment of PDI b'xa' Domain Construct
Deposition date:
2021-06-07
Original release date:
2021-06-17
Authors:
Pei, Yunshan; Liu, Xiaoli; cheng, Kai; Xu, Guohua; Jiang, Ling; Li, Conggang
Citation:

Citation: Pei, Yunshan; Liu, Xiaoli; Cheng, Kai; Xu, Guohua; Jiang, Ling; Li, Conggang. "Backbone resonance assignment of PDI b'xa' domain construct"  Biomol. NMR Assign. 15, 409-413 (2021).
PubMed: 34169459

Assembly members:

Assembly members:
entity_1, polymer, 249 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Data sets:
Data typeCount
13C chemical shifts659
15N chemical shifts211
1H chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDI b'xa'1

Entities:

Entity 1, PDI b'xa' 249 residues - Formula weight is not available

1   VALILEGLUPHETHRGLUGLNTHRALAPRO
2   LYSILEPHEGLYGLYGLUILELYSTHRHIS
3   ILELEULEUPHELEUPROLYSSERVALSER
4   ASPTYRASPGLYLYSLEUSERASNPHELYS
5   THRALAALAGLUSERPHELYSGLYLYSILE
6   LEUPHEILEPHEILEASPSERASPHISTHR
7   ASPASNGLNARGILELEUGLUPHEPHEGLY
8   LEULYSLYSGLUGLUCYSPROALAVALARG
9   LEUILETHRLEUGLUGLUGLUMETTHRLYS
10   TYRLYSPROGLUSERGLUGLULEUTHRALA
11   GLUARGILETHRGLUPHECYSHISARGPHE
12   LEUGLUGLYLYSILELYSPROHISLEUMET
13   SERGLNGLULEUPROGLUASPTRPASPLYS
14   GLNPROVALLYSVALLEUVALGLYLYSASN
15   PHEGLUASPVALALAPHEASPGLULYSLYS
16   ASNVALPHEVALGLUPHETYRALAPROTRP
17   CYSGLYHISCYSLYSGLNLEUALAPROILE
18   TRPASPLYSLEUGLYGLUTHRTYRLYSASP
19   HISGLUASNILEVALILEALALYSMETASP
20   SERTHRALAASNGLUVALGLUALAVALLYS
21   VALHISSERPHEPROTHRLEULYSPHEPHE
22   PROALASERALAASPARGTHRVALILEASP
23   TYRASNGLYGLUARGTHRLEUASPGLYPHE
24   LYSLYSPHELEUGLUSERGLYGLYGLNASP
25   GLYALAGLYHISHISHISHISHISHIS

Samples:

sample_1: PDI b'xa', [U-13C; U-15N; U-2H], 0.6 mM; sodium phosphate 20 mM; sodium chloride 200 mM; TCEP 5 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks