BMRB Entry 50962

Title:
Backbone 1H, 13C and 15N chemical shift assignment of C-terminal polybasic region of human Semaphorin 3A
Deposition date:
2021-06-07
Original release date:
2021-08-04
Authors:
Perez, Yolanda; Bonet, Roman; Corredor, Miriam; Domingo, Cecilia; Moure, Alejandra; Bujons, Jordi; Messeguer, Angel; Alfonso, Ignacio
Citation:

Citation: Perez, Yolanda; Bonet, Roman; Corredor, Miriam; Domingo, Cecilia; Moure, Alejandra; Messeguer, Angel; Bujons, Jordi; Alfonso, Ignacio. "Semaphorin 3A - Glycosaminoglycans interaction as therapeu-tic target for axonal regeneration"  Pharmaceuticals (Basel) 14, 906-906 (2021).
PubMed: 34577606

Assembly members:

Assembly members:
entity_1, polymer, 48 residues, 5915 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET45

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AVWKRDRKQRRQRPGHTPGN SNKWKHLQENKKGRNRRTHE FERAPRSV

Data sets:
Data typeCount
13C chemical shifts140
15N chemical shifts47
1H chemical shifts284

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal polybasic domain Sema3A1

Entities:

Entity 1, C-terminal polybasic domain Sema3A 48 residues - 5915 Da.

1   ALAVALTRPLYSARGASPARGLYSGLNARG
2   ARGGLNARGPROGLYHISTHRPROGLYASN
3   SERASNLYSTRPLYSHISLEUGLNGLUASN
4   LYSLYSGLYARGASNARGARGTHRHISGLU
5   PHEGLUARGALAPROARGSERVAL

Samples:

sample_1: C-terminal polybasic domain of Semaphorin 3A, [U-100% 13C; U-100% 15N], 1 mM; acetate 10 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 60 mM; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCACONHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5.7 - collection, data analysis

CcpNMR v2.4 - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Bruker 500 MHz Avance HDIII 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks