BMRB Entry 50951

Title:
MoPrP(89-230)_G2   PubMed: 34502545
Deposition date:
2021-05-20
Original release date:
2021-09-16
Authors:
Fridmanis, Jekabs; Jaudzems, Kristaps
Citation:

Citation: Fridmanis, Jekabs; Toleikis, Zigmantas; Sneideris, Tomas; Ziaunys, Mantas; Bobrovs, Raitis; Smirnovas, Vytautas; Jaudzems, Kristaps. "Aggregation Condition-Structure Relationship of Mouse Prion Protein Fibrils"  Int. J. Mol. Sci. 22, 9635-9635 (2021).

Assembly members:

Assembly members:
entity_1, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETB

Experimental source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETB

Data sets:
Data typeCount
13C chemical shifts216
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MoPrP(89-230)1

Entities:

Entity 1, MoPrP(89-230) 144 residues - Formula weight is not available

1   GLYSERGLYGLNGLYGLYGLYTHRHISASN
2   GLNTRPASNLYSPROSERLYSPROLYSTHR
3   ASNLEULYSHISVALALAGLYALAALAALA
4   ALAGLYALAVALVALGLYGLYLEUGLYGLY
5   TYRMETLEUGLYSERALAMETSERARGPRO
6   METILEHISPHEGLYASNASPTRPGLUASP
7   ARGTYRTYRARGGLUASNMETTYRARGTYR
8   PROASNGLNVALTYRTYRARGPROVALASP
9   GLNTYRSERASNGLNASNASNPHEVALHIS
10   ASPCYSVALASNILETHRILELYSGLNHIS
11   THRVALTHRTHRTHRTHRLYSGLYGLUASN
12   PHETHRGLUTHRASPVALLYSMETMETGLU
13   ARGVALVALGLUGLNMETCYSVALTHRGLN
14   TYRGLNLYSGLUSERGLNALATYRTYRASP
15   GLYARGARGSER

Samples:

sample_1: MoPrP(89-230), [U-100% 13C; U-100% 15N], 20 ± 5 mg; Guanidinium chloride 2 ± 0.005 M; sodium phosphate 50 ± 0.1 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_1
CONCAsample_1isotropicsample_conditions_1
DARRsample_1isotropicsample_conditions_1
2D ChhCsample_1isotropicsample_conditions_1
2D NhhCsample_1isotropicsample_conditions_1
2D 13C-detected NCAsample_1isotropicsample_conditions_1
2D 13C-detected NCOsample_1isotropicsample_conditions_1

Software:

CARA v1.9.2 - assignment

TOPSPIN v4.1.1 - Spectra acquisition

NMR spectrometers:

  • Bruker AVANCE III 800 MHz