BMRB Entry 50938

Title:
Natural Teixobactin - Lipid II complex
Deposition date:
2021-05-12
Original release date:
2022-05-11
Authors:
Shukla, Rhythm; Weingarth, Markus
Citation:

Citation: Shukla, Rhythm; Lavore, Francesca; Maity, Sourav; Derks, Maik; Jones, Chelsea; Vermeulen, Bram; Melcrova, Adela; Morris, Michael; Becker, Lea Marie; Wang, Xiaoqi; Kumar, Raj; Medeiros-Silva, Joao; van Beekveld, Roy; Bonvin, Alexandre; Lorent, Joseph; Lelli, Moreno; Nowick, James; MacGillavry, Harold; Peoples, Aaron; Spoering, Amy; Ling, Losee; Hughes, Dallas; Roos, Wouter; Breukink, Eefjan; Lewis, Kim; Weingarth, Markus. "Teixobactin kills bacteria by a two-pronged attack on the cell envelope"  Nature 608, 390-396 (2022).
PubMed: 35922513

Assembly members:

Assembly members:
Teixobactin, polymer, 11 residues, Formula weight is not available
Lipid II, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Eleftheria terrae   Taxonomy ID: 1597781   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Eleftheria terrae

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Teixobactin: XISXXISXAXI
Lipid II: XXAEKXX

Data sets:
Data typeCount
13C chemical shifts91
15N chemical shifts11
1H chemical shifts11

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Teixobactin1
2Lipid II2

Entities:

Entity 1, Teixobactin 11 residues - Formula weight is not available

N-[N-Methyl-D-Phe-Ile-Ser-D-Gln-D-aIle-Ile-Ser-]cyclo[D-Thr*-Ala-[3-(2-iminoimidazolidine-4beta-yl)-Ala-]-Ile-]

1   DPHILESERDGN28JILESERDTCALAENN
2   ILE

Entity 2, Lipid II 7 residues - Formula weight is not available

N-[(R)-2-[1-O-[[[[(3,7,11-Trimethyl-2,6,10-dodecatrienyl)oxy]phosphonyl]oxy]phosphonyl]- 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-alpha- D-glucopyranose-3-O-yl]propionyl]-L-Ala-D-gammaGlu-L-Lys-D-Ala-D-Ala-OH ester

1   ALADGLLYSALAXMUBNAG

Samples:

sample_1: Teixobactin, [U-13C; U-15N], 800 nmols; Lipid II, [U-13C; U-15N], 400 nmols; DOPC 9600 nmols

sample_conditions_1: ionic strength: 40 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D CCsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks