BMRB Entry 50934

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50934

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Title:
Assignment of HPr48, an N-terminal fragment of the disordered histidine phosphocarrier protein HPr
Deposition date:
2021-05-06
Original release date:
2021-09-17
Authors:
Neira, Jose Luis; Palomino-Schatzlein, Martina
Citation:

Citation: Neira, Jose Luis; Palomino-Schatzlein, Martina; Hurtado-Gomez, Estefania; Ortore, Maria G.; Falco, Alberto. "An N-terminal half fragment of the histidine phosphocarrier protein, HPr, is disordered but binds to HPr partners and shows antibacterial properties"  Biochim. Biophys. Acta Gen. Subj. 1865, 130015-130015 (2021).
PubMed: 34537288

Assembly members:

Assembly members:
entity_1, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LAERRVNVGWAEGLHARPAS IFVRAATATGVPVTIAKADG SPVNAASX

Data sets:
Data typeCount
13C chemical shifts90
15N chemical shifts45
1H chemical shifts278

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HPr481

Entities:

Entity 1, HPr48 48 residues - Formula weight is not available

1   LEUALAGLUARGARGVALASNVALGLYTRP
2   ALAGLUGLYLEUHISALAARGPROALASER
3   ILEPHEVALARGALAALATHRALATHRGLY
4   VALPROVALTHRILEALALYSALAASPGLY
5   SERPROVALASNALAALASERHSE

Samples:

sample_1: HPr48, [U-99% 13C; U-99% 15N], 490 uM; urea 0.5 M; TRIS 50 mM; sodium azide 2%

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks