BMRB Entry 50860

Title:
Tim9 reduced
Deposition date:
2021-03-27
Original release date:
2021-04-22
Authors:
Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul
Citation:

Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12"  Structure 29, 1065-1073 (2021).
PubMed: 33974880

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts217
15N chemical shifts75
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tim9 reduced1

Entities:

Entity 1, Tim9 reduced 87 residues - Formula weight is not available

1   METASPALALEUASNSERLYSGLUGLNGLN
2   GLUPHEGLNLYSVALVALGLUGLNLYSGLN
3   METLYSASPPHEMETARGLEUTYRSERASN
4   LEUVALGLUARGCYSPHETHRASPCYSVAL
5   ASNASPPHETHRTHRSERLYSLEUTHRASN
6   LYSGLUGLNTHRCYSILEMETLYSCYSSER
7   GLULYSPHELEULYSHISSERGLUARGVAL
8   GLYGLNARGPHEGLNGLUGLNASNALAALA
9   LEUGLYGLNGLYLEUGLYARG

Samples:

sample_1: Tim9, [U-13C; U-15N], 0.4 mM; MES 20 mM; sodium chloride 50 mM; TCEP 10 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

ANALYSIS - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks