BMRB Entry 50858

Title:
TIM91012
Deposition date:
2021-03-26
Original release date:
2021-04-22
Authors:
Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul
Citation:

Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12"  Structure 29, 1065-1073 (2021).
PubMed: 33974880

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available
entity_2, polymer, 93 residues, Formula weight is not available
entity_3, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Data sets:
Data typeCount
13C chemical shifts45
15N chemical shifts28
1H chemical shifts27

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tim91
2Tim102
3Tim123

Entities:

Entity 1, Tim9 87 residues - Formula weight is not available

1   METASPALALEUASNSERLYSGLUGLNGLN
2   GLUPHEGLNLYSVALVALGLUGLNLYSGLN
3   METLYSASPPHEMETARGLEUTYRSERASN
4   LEUVALGLUARGCYSPHETHRASPCYSVAL
5   ASNASPPHETHRTHRSERLYSLEUTHRASN
6   LYSGLUGLNTHRCYSILEMETLYSCYSSER
7   GLULYSPHELEULYSHISSERGLUARGVAL
8   GLYGLNARGPHEGLNGLUGLNASNALAALA
9   LEUGLYGLNGLYLEUGLYARG

Entity 2, Tim10 93 residues - Formula weight is not available

1   GLYSERPHELEUGLYPHEGLYGLYGLYGLN
2   PROGLNLEUSERSERGLNGLNLYSILEGLN
3   ALAALAGLUALAGLULEUASPLEUVALTHR
4   ASPMETPHEASNLYSLEUVALASNASNCYS
5   TYRLYSLYSCYSILEASNTHRSERTYRSER
6   GLUGLYGLULEUASNLYSASNGLUSERSER
7   CYSLEUASPARGCYSVALALALYSTYRPHE
8   GLUTHRASNVALGLNVALGLYGLUASNMET
9   GLNLYSMETGLYGLNSERPHEASNALAALA
10   GLYLYSPHE

Entity 3, Tim12 109 residues - Formula weight is not available

1   GLYSERPHEPHELEUASNSERLEUARGGLY
2   ASNGLNGLUVALSERGLNGLULYSLEUASP
3   VALALAGLYVALGLNPHEASPALAMETALA
4   SERTHRPHEASNASNILELEUSERTHRCYS
5   LEUGLULYSCYSILEPROHISGLUGLYPHE
6   GLYGLUPROASPLEUTHRLYSGLYGLUGLN
7   ALACYSILEASPARGCYSVALALALYSMET
8   HISTYRSERASNARGLEUILEGLYGLYPHE
9   VALGLNTHRARGGLYPHEGLYPROGLUASN
10   GLNLEUARGHISTYRSERARGPHEVALALA
11   LYSGLUILEALAASPASPSERLYSLYS

Samples:

sample_1: Tim12, [U-13C; U-15N], 0.2 mM; MES 20 mM; sodium chloride 50 mM; Tim9 0.2 mM; Tim10 0.2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

ANALYSIS - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks