BMRB Entry 50853

Name: Heme chemical shifts assignment of the PpcBF6L from Geobacter sulfurreducens
Published: 2021-07-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50853

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Heme chemical shifts assignment of the PpcBF6L from Geobacter sulfurreducens

Deposition date:

2021-03-26

Original release date:

2021-07-14

Authors:

Silva, Marta; Portela, Pilar; Salgueiro, Carlos

Citation:

Citation: Silva, Marta; Portela, Pilar; Salgueiro, Carlos. "Rational design of electron/proton transfer mechanisms in the exoelectrogenic bacteria Geobacter sulfurreducens" Biochem. J. 478, 2871-2887 (2021).
PubMed: 34190983

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available
entity_HEM, non-polymer, 616.487 Da.

Natural source:

Natural source: Common Name: Geobacter sulfurreducens Taxonomy ID: 35554 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacter sulfurreducens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pVA203_PpcBF6L

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ADTMTLTAKNGNVTFDHKKH QTIVPDCAVCHGKTPGKIEG FGKEMAHGKSCKGCHEEMKK GPTKCGECHKK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	36

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PpcBF6L_Polypeptide	1
2	HEM 1	2
3	HEM 2	2
4	HEM 3	2

Entities:

Entity 1, PpcBF6L_Polypeptide 71 residues - Formula weight is not available

1

ALA

ASP

THR

MET

THR

LEU

THR

ALA

LYS

ASN

2

GLY

ASN

VAL

THR

PHE

ASP

HIS

LYS

HIS

3

GLN

THR

ILE

VAL

PRO

ASP

CYS

ALA

VAL

CYS

4

HIS

GLY

LYS

THR

PRO

GLY

LYS

ILE

GLU

GLY

5

PHE

GLY

LYS

GLU

MET

ALA

HIS

GLY

LYS

SER

6

CYS

LYS

GLY

CYS

HIS

GLU

MET

LYS

7

GLY

PRO

THR

LYS

CYS

GLY

GLU

CYS

HIS

LYS

8

LYS

Entity 2, HEM 1 - C34 H32 Fe N4 O4 - 616.487 Da.

1

HEM

Samples:

sample_1: Cytochrome PpcBF6L from Geobacter sulfurreducens 1 mM; sodium phosphate 80 mM; sodium chloride 250 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 250 mM; pH: 8.0; pressure: 1 atm; temperature: 289.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - processing

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz