BMRB Entry 50842
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50842
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Boisvert, Olivier; Letourneau, Danny; Delattre, Patrick; Tremblay, Cynthia; Jolibois, Emilie; Montagne, Martin; Lavigne, Pierre. "Zinc Fingers 10 and 11 of Miz-1 undergo conformational exchange to achieve specific DNA binding" Structure 30, 623-636 (2022).
PubMed: 34963061
Assembly members:
entity_1, polymer, 85 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3a
Entity Sequences (FASTA):
entity_1: MKPYVCERCGKRFVQSSQLA
NHIRHHDNIRPYKCSVCSKA
FVNVGDLSKHIIIHTGEKPY
LCDKCGRGFNRVDNLRSHVK
TVHQG
| Data type | Count |
| 13C chemical shifts | 327 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 497 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | miz-1 10-12 | 1 |
| 2 | Zinc, 1 | 2 |
| 3 | Zinc, 2 | 2 |
| 4 | Zinc, 3 | 2 |
Entities:
Entity 1, miz-1 10-12 85 residues - Formula weight is not available
| 1 | MET | LYS | PRO | TYR | VAL | CYS | GLU | ARG | CYS | GLY | ||||
| 2 | LYS | ARG | PHE | VAL | GLN | SER | SER | GLN | LEU | ALA | ||||
| 3 | ASN | HIS | ILE | ARG | HIS | HIS | ASP | ASN | ILE | ARG | ||||
| 4 | PRO | TYR | LYS | CYS | SER | VAL | CYS | SER | LYS | ALA | ||||
| 5 | PHE | VAL | ASN | VAL | GLY | ASP | LEU | SER | LYS | HIS | ||||
| 6 | ILE | ILE | ILE | HIS | THR | GLY | GLU | LYS | PRO | TYR | ||||
| 7 | LEU | CYS | ASP | LYS | CYS | GLY | ARG | GLY | PHE | ASN | ||||
| 8 | ARG | VAL | ASP | ASN | LEU | ARG | SER | HIS | VAL | LYS | ||||
| 9 | THR | VAL | HIS | GLN | GLY |
Entity 2, Zinc, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Miz-1 ZF 10-12, [U-99% 13C; U-99% 15N], 500 ± 0.5 uM; ZINC ion 2.0 ± 0.1 mM; TCEP 2.0 ± 0.1 mM; Bis-Tris 50 ± 0.1 mM; potassium chloride 50 ± 0.1 mM
sample_conditions_1: ionic strength: 50.0 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA2 v2.3.2 - structure calculation
DANGLE v1.1 - data analysis
CcpNMR v2.4 - chemical shift assignment & data analysis
CNS v1.3 - refinement, structure calculation
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks