BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 50789

Title: 1H, 13C and 15N chemical shift assignments of the C-terminal domain of human UDP-Glucuronosyltransferase 2B7 (UGT2B7)   PubMed: 33870481

Deposition date: 2021-02-24 Original release date: 2021-05-18

Authors: Osborne, Michael; Rahardjo, Amanda; Volpon, Laurent; Borden, Katherine

Citation: Osborne, Michael; Rahardjo, Amanda; Volpon, Laurent; Borden, Katherine. "1H, 13C and 15N chemical shift assignments of the C-terminal domain of human UDP-Glucuronosyltransferase 2B7 (UGT2B7-C)"  Biomol. NMR Assignments ., .-. (2021).

Assembly members:
entity_1, polymer, 169 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28

Entity Sequences (FASTA):
entity_1: GSAKPLPKEMEDFVQSSGEN GVVVFSLGSMVSNMTEERAN VIASALAQIPQKVLWRFDGN KPDTLGLNTRLYKWIPQNDL LGHPKTRAFITHGGANGIYE AIYHGIPMVGIPLFADQPDN IAHMKARGAAVRVDFNTMSS TDLLNALKRVINDPSYKENV MKLSRIQHD

Data sets:
Data typeCount
13C chemical shifts606
15N chemical shifts145
1H chemical shifts825

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1c-terminus of UGT2B71

Entities:

Entity 1, c-terminus of UGT2B7 169 residues - Formula weight is not available

1   GLYSERALALYSPROLEUPROLYSGLUMET
2   GLUASPPHEVALGLNSERSERGLYGLUASN
3   GLYVALVALVALPHESERLEUGLYSERMET
4   VALSERASNMETTHRGLUGLUARGALAASN
5   VALILEALASERALALEUALAGLNILEPRO
6   GLNLYSVALLEUTRPARGPHEASPGLYASN
7   LYSPROASPTHRLEUGLYLEUASNTHRARG
8   LEUTYRLYSTRPILEPROGLNASNASPLEU
9   LEUGLYHISPROLYSTHRARGALAPHEILE
10   THRHISGLYGLYALAASNGLYILETYRGLU
11   ALAILETYRHISGLYILEPROMETVALGLY
12   ILEPROLEUPHEALAASPGLNPROASPASN
13   ILEALAHISMETLYSALAARGGLYALAALA
14   VALARGVALASPPHEASNTHRMETSERSER
15   THRASPLEULEUASNALALEULYSARGVAL
16   ILEASNASPPROSERTYRLYSGLUASNVAL
17   METLYSLEUSERARGILEGLNHISASP

Samples:

sample_1: C- terminus of UGT2B7, [U-100% 13C; U-100% 15N], 300 uM; sodium phosphate 50 mM; sodium chloride 300 mM; DTT 1 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.3 M; pH: 7.2; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRViewJ - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Related Database Links:

NCBI NP_009051

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts