BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50787

Title: Backbone chemical shift assignments of saccharomyces cerevisiae Ess1 Prolyl-isomerase C120S

Deposition date: 2021-02-23 Original release date: 2021-03-23

Authors: Zheng, Tongyin; Castaneda, Carlos

Citation: Zheng, Tongyin; Namitz, Kevin; Canning, Ashley; Alicea-Velazquez, Nilda; Castaneda, Carlos; Cosgrove, Michael; Hanes, Steven. "Structural basis for Ess1-dependent isomerization of the RNAPII CTD via a bivalent anchoring mechanism"  Commun. Biol. ., .-..

Assembly members:
entity_1, polymer, 174 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):
entity_1: GAMDMPSDVASSTGLPTPWT VRYSKSKKREYFFNPETKHS QWEEPEGTNKDQLHKHLRDH PVRVRCLHILIKHKDSRRPA SHRSENITISKQDATDELKT LITRLDDDSKTNSFEALAKE RSDSSSYKRGGDLGWFGRGE MQPSFEDAAFQLKVGEVSDI VESGSGVHVIKRVG

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts152
1H chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ess11

Entities:

Entity 1, Ess1 174 residues - Formula weight is not available

1   GLYALAMETASPMETPROSERASPVALALA
2   SERSERTHRGLYLEUPROTHRPROTRPTHR
3   VALARGTYRSERLYSSERLYSLYSARGGLU
4   TYRPHEPHEASNPROGLUTHRLYSHISSER
5   GLNTRPGLUGLUPROGLUGLYTHRASNLYS
6   ASPGLNLEUHISLYSHISLEUARGASPHIS
7   PROVALARGVALARGCYSLEUHISILELEU
8   ILELYSHISLYSASPSERARGARGPROALA
9   SERHISARGSERGLUASNILETHRILESER
10   LYSGLNASPALATHRASPGLULEULYSTHR
11   LEUILETHRARGLEUASPASPASPSERLYS
12   THRASNSERPHEGLUALALEUALALYSGLU
13   ARGSERASPSERSERSERTYRLYSARGGLY
14   GLYASPLEUGLYTRPPHEGLYARGGLYGLU
15   METGLNPROSERPHEGLUASPALAALAPHE
16   GLNLEULYSVALGLYGLUVALSERASPILE
17   VALGLUSERGLYSERGLYVALHISVALILE
18   LYSARGVALGLY

Samples:

sample_1: Peptidyl-prolyl cis-trans isomerase Ess1 C120S, [U-95% 13C; U-95% 15N], 500 uM; Na Phosphate 20 mM; NaCl 150 mM; TCEP 3 mM; NaN3 0.02%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.4.2 - chemical shift assignment, peak picking

TOPSPIN - collection

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Related Database Links:

UNP P22696

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts