BMRB Entry 50763

Title:
ARNT PAS-B Y456T sequence unfolded in 8M urea
Deposition date:
2021-02-12
Original release date:
2021-03-10
Authors:
Xu, Xingjian; Dikiy, Igor; Evans, Matthew; Marcelino, Leandro; Gardner, Kevin
Citation:

Citation: Xu, Xingjian; Dikiy, Igor; Evans, Matthew; Marcelino, Leandro; Gardner, Kevin. "Fragile protein folds: sequence and environmental factors affecting the equilibrium of two interconverting, stably folded protein conformations"  Magn. Reson. 2, 63-76 (2021).

Assembly members:

Assembly members:
entity_1, polymer, 119 residues, 13880.67 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis-parallel

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts112
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ARNT PAS-B Y456T urea unfolded1

Entities:

Entity 1, ARNT PAS-B Y456T urea unfolded 119 residues - 13880.67 Da.

N-terminal 4 residues (GAMD) are vector-derived; remaining sequence is residues 356-470 from human ARNT protein.

1   GLYALAMETASPASNVALCYSGLNPROTHR
2   GLUPHEILESERARGHISASNILEGLUGLY
3   ILEPHETHRPHEVALASPHISARGCYSVAL
4   ALATHRVALGLYTYRGLNPROGLNGLULEU
5   LEUGLYLYSASNILEVALGLUPHECYSHIS
6   PROGLUASPGLNGLNLEULEUARGASPSER
7   PHEGLNGLNVALVALLYSLEULYSGLYGLN
8   VALLEUSERVALMETPHEARGPHEARGSER
9   LYSASNGLNGLUTRPLEUTRPMETARGTHR
10   SERSERPHETHRPHEGLNASNPROTYRSER
11   ASPGLUILEGLUTHRILEILECYSTHRASN
12   THRASNVALLYSASNSERSERGLNGLU

Samples:

sample_1: ARNT PAS-B Y456T, [U-99% 13C; U-99% 15N], 500 uM; urea 8 M; TRIS 50 mM; sodium chloride 20 mM; beta-mercaptoethanol 5 mM

sample_conditions_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMRPipe - processing

VNMRj - collection

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

UNP P27540
AlphaFold Q8NDC7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks