BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50752

Title: Backbone chemical shift assignments of PSD-95 N-terminal domain   PubMed: 33929702

Deposition date: 2021-02-08 Original release date: 2021-05-18

Authors: Zhang, Yonghong

Citation: Zhang, Yonghong; Hell, Johannes; Ames, James. "Chemical shift assignments of the N-terminal domain of PSD95 (PSD95-NT)"  Biomolecular NMR Assignments ., .-..

Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: E. coli   Vector: pGEX-4T3

Entity Sequences (FASTA):
entity_1: GSMDCLCIVTTKKYRYQDED TPPLEHSPAHLPNQANSPPV IVNTDTLEAPGYELQVNGTE GEMEYEEITLERG

Data sets:
Data typeCount
13C chemical shifts197
15N chemical shifts62
1H chemical shifts256

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PSD-95NTD1

Entities:

Entity 1, PSD-95NTD 73 residues - Formula weight is not available

1   GLYSERMETASPCYSLEUCYSILEVALTHR
2   THRLYSLYSTYRARGTYRGLNASPGLUASP
3   THRPROPROLEUGLUHISSERPROALAHIS
4   LEUPROASNGLNALAASNSERPROPROVAL
5   ILEVALASNTHRASPTHRLEUGLUALAPRO
6   GLYTYRGLULEUGLNVALASNGLYTHRGLU
7   GLYGLUMETGLUTYRGLUGLUILETHRLEU
8   GLUARGGLY

Samples:

sample_1: PSD95NT-1, [U-99% 13C; U-99% 15N], 0.1 mM

sample_conditions_1: ionic strength: 36 mM; pH: 5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignments

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts