BMRB Entry 50750

Name: 1H, 13C and 15N chemical shift backbone resonance NMR assignment of Tobacco Calmoduline 2
Published: 2021-02-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50750

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift backbone resonance NMR assignment of Tobacco Calmoduline 2

Deposition date:

2021-02-08

Original release date:

2021-02-17

Authors:

Trivelli, Xavier; Boll, Emmanuelle; Cantrelle, Francois-Xavier; Lamotte, Olivier; Wendehenne, David; Aime, Sebastien

Citation:

Citation: Boll, Emmanuelle; Cantrelle, Francois-Xavier; Lamotte, Olivier; Aime, Sebastien; Wendehenne, David; Trivelli, Xavier. "1H, 13C and 15N chemical shift backbone resonance NMR assignment of tobacco calmodulin 2" Biomol. NMR Assignments 16, 63-66 (2022).
PubMed: 35020112

Assembly members:

Assembly members:
entity_1, polymer, 149 residues, 16909.6094 Da.

Natural source:

Natural source: Common Name: Tobacco nicotiana Taxonomy ID: 4085 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Nicotiana Tobacco nicotiana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAEQLTEEQIAEFKEAFSLF DKDGDGCITTKELGTVMRSL GQNPTEAELQDMISEVDADQ NGTIDFPEFLNLMARKMKDT DSEEELKEAFKVFDKDQNGF ISAAELRHVMTNLGEKLTDE EVDEMIREADIDGDGQVNYE EFVRMMLAK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	439
15N chemical shifts	152
1H chemical shifts	161

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CaM	1

Entities:

Entity 1, CaM 149 residues - 16909.6094 Da.

1

MET

ALA

GLU

GLN

LEU

THR

GLU

GLN

ILE

2

ALA

GLU

PHE

LYS

GLU

ALA

PHE

SER

LEU

PHE

3

ASP

LYS

ASP

GLY

ASP

GLY

CYS

ILE

THR

4

LYS

GLU

LEU

GLY

THR

VAL

MET

ARG

SER

LEU

5

GLY

GLN

ASN

PRO

THR

GLU

ALA

GLU

LEU

GLN

6

ASP

MET

ILE

SER

GLU

VAL

ASP

ALA

ASP

GLN

7

ASN

GLY

THR

ILE

ASP

PHE

PRO

GLU

PHE

LEU

8

ASN

LEU

MET

ALA

ARG

LYS

MET

LYS

ASP

THR

9

ASP

SER

GLU

LEU

LYS

GLU

ALA

PHE

10

LYS

VAL

PHE

ASP

LYS

ASP

GLN

ASN

GLY

PHE

11

ILE

SER

ALA

GLU

LEU

ARG

HIS

VAL

MET

12

THR

ASN

LEU

GLY

GLU

LYS

LEU

THR

ASP

GLU

13

GLU

VAL

ASP

GLU

MET

ILE

ARG

GLU

ALA

ASP

14

ILE

ASP

GLY

ASP

GLY

GLN

VAL

ASN

TYR

GLU

15

GLU

PHE

VAL

ARG

MET

LEU

ALA

LYS

Samples:

sample_1: CaM, [U-98% 13C; U-98% 15N], 0.15 mM; Hepes 100 mM; KCl 100 mM; EGTA 10 mM; CaCl2 7.94 mM; NTA 10 mM

sample_conditions_1: ionic strength: 0.135 M; pH: 7.400; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
Expt_7 (H[N[co[{CA\|ca[C]}]]])	sample_1	isotropic	sample_conditions_1
Expt_14 (H[N[ca[CO]]])	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.5 - Assignment

MddNMR v2.5 - processing

NMRPipe vX.2 - processing

TOPSPIN v4.0.7 - acquisition

NMR spectrometers:

Bruker AVANCE NEO 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks