BMRB Entry 50746

Title:
1H, 13C and 15N assignment of the flexible C-terminus of alpha-synuclein bound to lipid vesicles   PubMed: 33797711
Deposition date:
2021-02-03
Original release date:
2021-04-06
Authors:
Medeiros, Justin; Bamm, Vladimir; Jany, Catherine; Coackley, Carla; Ward, Meaghan; Harauz, George; Ryan, Scott; Ladizhansky, Vladimir
Citation:

Citation: Medeiros, Justin; Bamm, Vladimir; Jany, Catherine; Coackley, Carla; Ward, Meaghan; Harauz, George; Ryan, Scott; Ladizhansky, Vladimir. "Partial magic angle spinning NMR 1H, 13C, 15N resonance assignments of the flexible regions of a monomeric alpha-synuclein: conformation of C-terminus in the lipid-bound and amyloid fibril states"  Biomol. NMR Assignments 15, 297-303 (2021).

Assembly members:

Assembly members:
entity_1, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Data sets:
Data typeCount
13C chemical shifts118
15N chemical shifts34
1H chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aSyn-WT1

Entities:

Entity 1, aSyn-WT 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: Alpha-Synuclein, [U-99% 13C; U-99% 15N], 20.75 uM; potassium phosphate 10 mM; DOPA:DOPC (41:59) 2.075 mM; H20 100%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 13C/13C TOBSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA v1.9 - chemical shift assignments

NMRPipe - processing

TOPSPIN v3.5 - collection

NMR spectrometers:

  • Bruker AVANCE III 800.230 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks