BMRB Entry 50738

Title:
Smad2 interdomain linker
Deposition date:
2021-01-27
Original release date:
2021-09-14
Authors:
Gomes, Tiago
Citation:

Citation: Gomes, Tiago; Martin-Malpartida, Pau; Ruiz, Lidia; Aragona, Eric; Cordeiro, Tiago; Macias, Maria. "Conformational landscape of multidomain SMAD proteins"  Comp. Struc. Biotech. J. 19, 5210-5224 (2021).
PubMed: 34630939

Assembly members:

Assembly members:
entity_1, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PETM11

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts65
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Smad2linker1

Entities:

Entity 1, Smad2linker 107 residues - Formula weight is not available

1   METALAHISHISHISHISHISHISSERSER
2   GLYLEUGLUVALLEUPHEGLNGLYPROLEU
3   PROPROVALLEUVALPROARGHISTHRGLU
4   ILELEUTHRGLULEUPROPROLEUASPASP
5   TYRTHRHISSERILEPROGLUASNTHRASN
6   PHEPROALAGLYILEGLUPROGLNSERASN
7   TYRILEPROGLUTHRPROPROPROGLYTYR
8   ILESERGLUASPGLYGLUTHRSERASPGLN
9   GLNLEUASNGLNSERMETASPTHRGLYSER
10   PROALAGLULEUSERPROTHRTHRLEUSER
11   PROVALASNHISSERLEUASP

Samples:

sample_1: s4linker, [U-100% 13C; U-100% 15N], 400 uM

sample_conditions_1: ionic strength: 0.15 M; pH: 7; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
HNCACONHsample_1isotropicsample_conditions_1
HNCANHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks