BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50733

Title: NN206* (P22A and M85A; hereafter NN206*)   PubMed: 33929321

Deposition date: 2021-01-25 Original release date: 2021-05-18

Authors: Tzeng, Shiou-Ru

Citation: Tzeng, Shiou-Ru; Tseng, Yin-Chu; Lin, Chien-Chu; Hsu, Chia-Ying; Huang, Shing-Jong; Kuo, Yi-Ting; Chang, Chung-I. "Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease"  elife 10, e64056-e64056 (2021).

Assembly members:
entity_1, polymer, 206 residues, Formula weight is not available

Natural source:   Common Name: Meiothermus taiwanensis   Taxonomy ID: 172827   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Meiothermus taiwanensis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-206*

Entity Sequences (FASTA):
entity_1: MRLELPVIPLRNTVILPHTT TAVDVGRAKSKRAVEEAMGA DRLIFLVAQRDPEVDDPAPD DLYTWGVQAVVKQAMRLPDG TLQVAVEARARAQVTDYIPG PYLRARGEVFSEIFPIDEAV VRVLVEELKEAFEKYVANHK SLRLDRYQLEAVKGTSDPAM LADTIAYHATWTVAEKQEIL ELTDLEARLKKVLGLLSRDL ERFELD

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts187
1H chemical shifts187

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NN206* (P22A and M85A)1

Entities:

Entity 1, NN206* (P22A and M85A) 206 residues - Formula weight is not available

1   METARGLEUGLULEUPROVALILEPROLEU
2   ARGASNTHRVALILELEUPROHISTHRTHR
3   THRALAVALASPVALGLYARGALALYSSER
4   LYSARGALAVALGLUGLUALAMETGLYALA
5   ASPARGLEUILEPHELEUVALALAGLNARG
6   ASPPROGLUVALASPASPPROALAPROASP
7   ASPLEUTYRTHRTRPGLYVALGLNALAVAL
8   VALLYSGLNALAMETARGLEUPROASPGLY
9   THRLEUGLNVALALAVALGLUALAARGALA
10   ARGALAGLNVALTHRASPTYRILEPROGLY
11   PROTYRLEUARGALAARGGLYGLUVALPHE
12   SERGLUILEPHEPROILEASPGLUALAVAL
13   VALARGVALLEUVALGLUGLULEULYSGLU
14   ALAPHEGLULYSTYRVALALAASNHISLYS
15   SERLEUARGLEUASPARGTYRGLNLEUGLU
16   ALAVALLYSGLYTHRSERASPPROALAMET
17   LEUALAASPTHRILEALATYRHISALATHR
18   TRPTHRVALALAGLULYSGLNGLUILELEU
19   GLULEUTHRASPLEUGLUALAARGLEULYS
20   LYSVALLEUGLYLEULEUSERARGASPLEU
21   GLUARGPHEGLULEUASP

Samples:

sample_1: NN206* (P22A and M85A; hereafter NN206*), [U-99% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVIII-800 800MHz Super-Conducting Magnet - 18.79 Tesla MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts