BMRB Entry 50725

Title:
backbone assignments of SARS-CoV-2 Nsp9   PubMed: 34369011
Deposition date:
2021-01-21
Original release date:
2021-02-22
Authors:
El-Kamand, Serene; Du Plessis, Mar-Dean; Cubeddu, Liza; Gamsjaeger, Roland
Citation:

Citation: El-Kamand, Serene; Du Plessis, Mar-Dean; Breen, Natasha; Johnson, Lexie; Beard, Samuel; Kwan, Ann; Richard, Derek; Cubeddu, Liza; Gamsjaeger, Roland. "A distinct ssDNA/RNA binding interface in the Nsp9 protein from SARS-CoV-2"  Proteins 90, 176-185 (2022).

Assembly members:

Assembly members:
entity_1, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Experimental source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Data sets:
Data typeCount
13C chemical shifts172
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nsp91

Entities:

Entity 1, Nsp9 113 residues - Formula weight is not available

1   ASNASNGLULEUSERPROVALALALEUARG
2   GLNMETSERCYSALAALAGLYTHRTHRGLN
3   THRALACYSTHRASPASPASNALALEUALA
4   TYRTYRASNTHRTHRLYSGLYGLYARGPHE
5   VALLEUALALEULEUSERASPLEUGLNASP
6   LEULYSTRPALAARGPHEPROLYSSERASP
7   GLYTHRGLYTHRILETYRTHRGLULEUGLU
8   PROPROCYSARGPHEVALTHRASPTHRPRO
9   LYSGLYPROLYSVALLYSTYRLEUTYRPHE
10   ILELYSGLYLEUASNASNLEUASNARGGLY
11   METVALLEUGLYSERLEUALAALATHRVAL
12   ARGLEUGLN

Samples:

sample_1: Nsp9, [U-100% 13C; U-100% 15N], 0.935 mM; sodium phosphate 25 mM; NaCl 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks