BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50718

Title: Monomeric phospholamban in oriented bicelles   PubMed: 33978571

Deposition date: 2021-01-18 Original release date: 2021-05-18

Authors: Weber, Daniel; Sanz-Hernandez, Maximo; Uddigiri, Venkateswara Reddy; Wang, Songlin; Larsen, Erik; Gopinath, Tata; Gustavsson, Martin; Cornea, Razvan; Thomas, David; De Simone, Alfonso; Veglia, Gianluigi

Citation: Weber, Daniel; Reddy, U Venkateswara; Wang, Songlin; Larsen, Erik; Gopinath, Tata; Gustavsson, Martin; Cornea, Razvan; Thomas, David; De Simone, Alfonso; Veglia, Gianluigi. "Structural basis for allosteric control of the SERCA-Phospholamban membrane complex by Ca 2+ and phosphorylation"  Elife 10, e66226-e66226 (2021).

Assembly members:
entity_1, polymer, 53 residues, 6166 Da.

Natural source:   Common Name: rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMal-c2x

Entity Sequences (FASTA):
entity_1: AMEKVQYLTRSAIRRASTIE MPQQARQNLQNLFINFALIL IFLLLIAIIVMLL

Data sets:
Data typeCount
15N chemical shifts22
dipolar coupling values22

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PLN1

Entities:

Entity 1, PLN 53 residues - 6166 Da.

Monomeric cysteine-null mutant (C36A/C41F/C46A). N-terminal Ala0 left over from cleavage of MBP fusion protein.

1   ALAMETGLULYSVALGLNTYRLEUTHRARG
2   SERALAILEARGARGALASERTHRILEGLU
3   METPROGLNGLNALAARGGLNASNLEUGLN
4   ASNLEUPHEILEASNPHEALALEUILELEU
5   ILEPHELEULEULEUILEALAILEILEVAL
6   METLEULEU

Samples:

sample_1: Phospholamban (PLN-AFA), [U-15N], 2.3 mM; HEPES 20 mM; potassium chloride 100 mM; sodium azide 1 mM; glycerol 2.5%; 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) 340 mM; 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) 85 mM; 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) 106 mM; 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (PE-DTPA) 6.4 mM; Ytterbium(III) chloride 5 mM

sample_conditions_1: pH: 4.2; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D NH SLFsample_1anisotropicsample_conditions_1

Software:

NMRPipe - processing

VNMRj - collection

NMRFAM-SPARKY - data analysis

NMR spectrometers:

  • Varian VXRS 700 MHz

Related Database Links:

UNP P61015
PDB