BMRB Entry 50714

Title:
Solution NMR backbone assignments of the N-terminal Za domain from Homo Sapiens ADAR1p150.
Deposition date:
2021-01-16
Original release date:
2021-03-21
Authors:
Nichols, Parker; Henen, Morks; Vicens, Quentin; Vogeli, Beat
Citation:

Citation: Nichols, Parker; Henen, Morks; Vicens, Quentin; Vogeli, Beat. "Solution NMR backbone assignments of the N-terminal Zalpha-linker-Zbeta segment from Homo sapiens ADAR1p150"  Biomol. NMR Assign. 15, 273-279 (2021).
PubMed: 33742389

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet-28a(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts120
15N chemical shifts124
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Za1

Entities:

Entity 1, Za 64 residues - Formula weight is not available

1   METGLUGLNARGILELEULYSPHELEUGLU
2   GLULEUGLYGLUGLYLYSALATHRTHRALA
3   HISASPLEUSERGLYLYSLEUGLYTHRPRO
4   LYSLYSGLUILEASNARGVALLEUTYRSER
5   LEUALALYSLYSGLYLYSLEUGLNLYSGLU
6   ALAGLYTHRPROPROLEUTRPLYSILEALA
7   VALSERTHRGLN

Samples:

sample_1: Za, [U-100% 13C; U-100% 15N], 2 mM; potassium phosphate 20 mM; NaCl 25 mM

sample_2: Za, [U-100% 13C; U-100% 15N], 2 mM; potassium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2

Software:

NMRDraw v10.9 - processing

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Bruker Ultrashield 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks