BMRB Entry 50712

Title:
1H, 13C and 15N backbone resonance assignment of HIV-1 Gag(276-432) encompassing the C-terminal domain of the capsid protein, the spacer peptide 1 and the nucleocapsid protein
Deposition date:
2021-01-15
Original release date:
2021-03-29
Authors:
CHEN, Xiaowei; Coric, Pascale; Bouaziz, Serge
Citation:

Citation: Chen, Xiaowei; Coric, Pascale; Bouaziz, Serge. "1H, 13C and 15N backbone resonance assignment of HIV-1 Gag (276-432) encompassing the C-terminal domain of the capsid protein, the spacer peptide 1 and the nucleocapsid protein"  Biomol. NMR Assignments 15, 267-271 (2021).
PubMed: 33754285

Assembly members:

Assembly members:
entity_1, polymer, 157 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLATE11

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts137
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CActdSP1NC1

Entities:

Entity 1, CActdSP1NC 157 residues - Formula weight is not available

1   METTYRSERPROTHRSERILELEUASPILE
2   ARGGLNGLYPROLYSGLUPROPHEARGASP
3   TYRVALASPARGPHETYRLYSTHRLEUARG
4   ALAGLUGLNALASERGLNGLUVALLYSASN
5   ALAALATHRGLUTHRLEULEUVALGLNASN
6   ALAASNPROASPCYSLYSTHRILELEULYS
7   ALALEUGLYPROGLYALATHRLEUGLUGLU
8   METMETTHRALACYSGLNGLYVALGLYGLY
9   PROGLYHISLYSALAARGVALLEUALAGLU
10   ALAMETSERGLNVALTHRASNPROALATHR
11   ILEMETILEGLNLYSGLYASNPHEARGASN
12   GLNARGLYSTHRVALLYSCYSPHEASNCYS
13   GLYLYSGLUGLYHISILEALALYSASNCYS
14   ARGALAPROARGLYSLYSGLYCYSTRPLYS
15   CYSGLYLYSGLUGLYHISGLNMETLYSASP
16   CYSTHRGLUARGGLNALAASN

Samples:

sample_1: CActdSP1NC, [U-99% 13C; U-99% 15N], 0.5 mM; beta-mercaptoethanol 0.1 mM; sodium acetate, [U-99% 2H], 25 mM; sodium chloride 25 mM; zinc chloride 0.1 mM; D2O, [U-99% 2H], 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N SOFAST-HMQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis - chemical shift assignment

TOPSPIN - collection

TALOS-N - secondary structure prediction

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks