BMRB Entry 50709

Title:
RGG Mini
Deposition date:
2021-01-15
Original release date:
2021-11-14
Authors:
Mompean, Miguel; Laurents, Douglas
Citation:

Citation: Mompean, Miguel; Oroz, Javier; Laurents, Douglas. "Do polyproline II helix associations modulate biomolecular condensates?"  FEBS Open Bio 11, 2390-2399 (2021).
PubMed: 33934561

Assembly members:

Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XRGGYGGRGGWGGRGGYX

Data sets:
Data typeCount
13C chemical shifts82
15N chemical shifts1
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RGG mini1

Entities:

Entity 1, RGG mini 18 residues - Formula weight is not available

The peptide is N-acetylated and C-amidated.

1   ACEARGGLYGLYTYRGLYGLYARGGLYGLY
2   TRPGLYGLYARGGLYGLYTYRNH2

Samples:

sample_1: RGG mini 6.1 ± 0.2 mM; DSS 0.05 ± 0.005 mM

sample_conditions_1: ionic strength: 10 mM; pH: 2.7; pressure: 0.92 atm; temperature: 278.2 K

sample_conditions_2: ionic strength: 10 mM; pH: 9.95; pressure: 0.92 atm; temperature: 278.2 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_2
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_2
2D 1H-1H COSYsample_1isotropicsample_conditions_2
2D 1H-1H TOCSYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_2

Software:

TOPSPIN v4.0.8 - collection, processing

NMRFAM-SPARKY v3.13 - chemical shift assignment, peak picking

CYANA - structure solution

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz
  • Bruker AVANCE NEO 800 MHz