BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50702

Title: Degron-tagged Ig2D5   PubMed: 33929321

Deposition date: 2021-01-08 Original release date: 2021-05-18

Authors: Tzeng, Shiou-Ru

Citation: Tzeng, Shiou-Ru; Tseng, Yin-Chu; Lin, Chien-Chu; Hsu, Chia-Ying; Huang, Shing-Jong; Kuo, Yi-Ting; Chang, Chung-I. "Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease"  elife 10, e64056-e64056 (2021).

Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: Dictyostelium discoideum   Taxonomy ID: 44689   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dictyostelium discoideum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_Ig2D5_S20

Entity Sequences (FASTA):
entity_1: GHMTVKPAPSAEHSYAEGEG LVKVFDNAPAEFTIFAVDTK GVARTDGGDPFEVAINGPDG LVVDAKVTDNNDGTYGVVYD APVEGNYNVNVTLRGNPIKN MPIDVKCIEGASSHATRQLS GLKIHSNLYH

Data sets:
Data typeCount
13C chemical shifts215
15N chemical shifts115
1H chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Degron tag Sul201

Entities:

Entity 1, Degron tag Sul20 130 residues - Formula weight is not available

A degron-tagged Ig2D5 is a chimeric protein: Sul20 (the C-terminal 20 residues of SulA; namely Sul20) is appended to the C terminus of a protein substrate Ig2D5 (domains 5 of the gelation factor from Dictyostelium discoideum. The sequence of Ig2D5: GHMTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGGDPFEVAINGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEG The sequence of Sul20: ASSHATRQLSGLKIHSNLYH

1   GLYHISMETTHRVALLYSPROALAPROSER
2   ALAGLUHISSERTYRALAGLUGLYGLUGLY
3   LEUVALLYSVALPHEASPASNALAPROALA
4   GLUPHETHRILEPHEALAVALASPTHRLYS
5   GLYVALALAARGTHRASPGLYGLYASPPRO
6   PHEGLUVALALAILEASNGLYPROASPGLY
7   LEUVALVALASPALALYSVALTHRASPASN
8   ASNASPGLYTHRTYRGLYVALVALTYRASP
9   ALAPROVALGLUGLYASNTYRASNVALASN
10   VALTHRLEUARGGLYASNPROILELYSASN
11   METPROILEASPVALLYSCYSILEGLUGLY
12   ALASERSERHISALATHRARGGLNLEUSER
13   GLYLEULYSILEHISSERASNLEUTYRHIS

Samples:

sample_1: A degron-tagged Ig2D5, [U-100% 13C; U-100% 15N], 0.3 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVIII-800 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts