BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50698

Title: Domains 5 of the gelation factor from Dictyostelium discoideum   PubMed: 33929321

Deposition date: 2021-01-07 Original release date: 2021-05-18

Authors: Tzeng, Shiou-Ru

Citation: Tzeng, Shiou-Ru; Tseng, Yin-Chu; Lin, Chien-Chu; Hsu, Chia-Ying; Huang, Shing-Jong; Kuo, Yi-Ting; Chang, Chung-I. "Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease"  elife 10, e64056-e64056 (2021).

Assembly members:
entity_1, polymer, 110 residues, Formula weight is not available

Natural source:   Common Name: Dictyostelium discoideum   Taxonomy ID: 44689   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dictyostelium discoideum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-Ig2D5

Entity Sequences (FASTA):
entity_1: GHMTVKPAPSAEHSYAEGEG LVKVFDNAPAEFTIFAVDTK GVARTDGGDPFEVAINGPDG LVVDAKVTDNNDGTYGVVYD APVEGNYNVNVTLRGNPIKN MPIDVKCIEG

Data sets:
Data typeCount
13C chemical shifts181
15N chemical shifts97
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ig2D51

Entities:

Entity 1, Ig2D5 110 residues - Formula weight is not available

1   GLYHISMETTHRVALLYSPROALAPROSER
2   ALAGLUHISSERTYRALAGLUGLYGLUGLY
3   LEUVALLYSVALPHEASPASNALAPROALA
4   GLUPHETHRILEPHEALAVALASPTHRLYS
5   GLYVALALAARGTHRASPGLYGLYASPPRO
6   PHEGLUVALALAILEASNGLYPROASPGLY
7   LEUVALVALASPALALYSVALTHRASPASN
8   ASNASPGLYTHRTYRGLYVALVALTYRASP
9   ALAPROVALGLUGLYASNTYRASNVALASN
10   VALTHRLEUARGGLYASNPROILELYSASN
11   METPROILEASPVALLYSCYSILEGLUGLY

Samples:

sample_1: Ig2D5, [U-99% 13C; U-99% 15N], 0.6 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVIII-800 800MHz Super-Conducting Magnet - 18.79 Tesla MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts