BMRB Entry 50688

Name: Solution NMR structure of mouse N4BP1 CUE domain
Published: 2021-02-02

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50688

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution NMR structure of mouse N4BP1 CUE domain

Deposition date:

2021-01-02

Original release date:

2021-02-02

Authors:

Song, Wei; Garnett, James; Stieglitz, Benjamin

Citation:

Citation: Song, Wei; Garnett, James; Stieglitz, Benjamin. "Assigned Chemical Shifts for mouse N4BP1 CUE domain" .

Assembly members:

Assembly members:
entity_1, polymer, 44 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET47b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ETSELREALLKIFPDSEQKL KIDQILAAHPYMKDLNALSA LVLD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	191
15N chemical shifts	41
1H chemical shifts	314

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N4BP1 CUE domain	1

Entities:

Entity 1, N4BP1 CUE domain 44 residues - Formula weight is not available

1

GLU

THR

SER

GLU

LEU

ARG

GLU

ALA

LEU

2

LYS

ILE

PHE

PRO

ASP

SER

GLU

GLN

LYS

LEU

3

LYS

ILE

ASP

GLN

ILE

LEU

ALA

HIS

PRO

4

TYR

MET

LYS

ASP

LEU

ASN

ALA

LEU

SER

ALA

5

LEU

VAL

LEU

ASP

Samples:

sample_1: N4BP1 CUE domain, [U-100% 13C; U-100% 15N], 500 uM; D2O 10%; sodium phosphate 22 mM; sodium chloride 55 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 7; pressure: 1 atm; temperature: 303.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

CS-Rosetta - structure solution

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks