BMRB Entry 50683

Name: Resonance assignment of human STIM1 CC3 fragment
Published: 2021-08-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50683

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignment of human STIM1 CC3 fragment

Deposition date:

2020-12-22

Original release date:

2021-08-23

Authors:

Gupta, Agrim; Kitzler, Christian; Rathner, Petr; Romanin, Christoph; Mueller, Norbert

Citation:

Citation: Gupta, Agrim; Kitzler, Christian Manuel; Rathner, Petr; Fahrner, Marc; Grabmayr, Herwig; Rathner, Adriana; Romanin, Christoph; Muller, Norbert. "Resonance assignment of coiled-coil 3 (CC3) domain of human STIM1" Biomol. NMR Assignments 15, 433-439 (2021).
PubMed: 34417953

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX 4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NTLFGTFHVAHSSSLDDVDH KILTAKQALSEVTAALRERL HRW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	166
15N chemical shifts	46
1H chemical shifts	258

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	STIM1 CC3 wild type	1

Entities:

Entity 1, STIM1 CC3 wild type 43 residues - Formula weight is not available

1

ASN

THR

LEU

PHE

GLY

THR

PHE

HIS

VAL

ALA

2

HIS

SER

LEU

ASP

VAL

ASP

HIS

3

LYS

ILE

LEU

THR

ALA

LYS

GLN

ALA

LEU

SER

4

GLU

VAL

THR

ALA

LEU

ARG

GLU

ARG

LEU

5

HIS

ARG

TRP

Samples:

sample_1: Bis-Tris 20 mM; H2O, [U-99% 2H], 10 % w/v; H2O 90 % w/v; Triflouroethanol 17.5 % v/v; STIM1 CC3, [U-95% 13C; U-95% 15N], 0.5 mM

sample_2: Bis-Tris 20 mM; H2O, [U-99% 2H], 10 % w/v; H2O 90 % w/v; Triflouroethanol, [U-99% 2H], 17.5 % v/v; STIM1 CC3, [U-95% 13C; U-95% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1

Software:

CARA v1.8.4.2 - chemical shift assignment

TOPSPIN v3.5, 3.6 - collection

NMR spectrometers:

Bruker AVANCE III 700MHz MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks