BMRB Entry 50681

Title:
1H, 13C, 15N reduced mitoNEET assignment
Deposition date:
2020-12-22
Original release date:
2022-02-10
Authors:
Camponeschi, Francesca; Gallo, Angelo; Piccioli, Mario; Banci, Lucia
Citation:

Citation: Camponeschi, Francesca; Gallo, Angelo; Piccioli, Mario; Banci, Lucia. "The long-standing relationship between paramagnetic NMR and iron-sulfur proteins: the mitoNEET example. An old method for new stories or the other way around?"  Magn. Reson. 2, 203-221 (2021).

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, Formula weight is not available
entity_FES, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151-D/TOPO

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts44
1H chemical shifts286

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mitoNEET1
2FES2

Entities:

Entity 1, mitoNEET 83 residues - Formula weight is not available

The real sequence start from residue 7 and it should be translated to number 32

1   GLYILEASPPROPHEMETLYSARGPHETYR
2   VALLYSASPHISARGASNLYSALAMETILE
3   ASNLEUHISILEGLNLYSASPASNPROLYS
4   ILEVALHISALAPHEASPMETGLUASPLEU
5   GLYASPLYSALAVALTYRCYSARGCYSTRP
6   ARGSERLYSLYSPHEPROPHECYSASPGLY
7   ALAHISTHRLYSHISASNGLUGLUTHRGLY
8   ASPASNVALGLYPROLEUILEILELYSLYS
9   LYSGLUTHR

Entity 2, FES - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: mitoNEET protein cytosolic region, [U-13C; U-15N], 0.5 mM; D2O, [U-2H], 10%; H2O 90%; potassium phosphate 50 mM; sodium dithionite 10 mM

sample_conditions_1: ionic strength: 60 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection

TOPSPIN v3.2 - processing

CARA v1.9.2a4 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 500 MHz

Related Database Links:

UNP Q9NZ45
AlphaFold Q1X902

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks