BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50650

Title: human Angiogenin pH 6.3 35oC   PubMed: 33535464

Deposition date: 2020-12-15 Original release date: 2021-03-15

Authors: Fagagnini, Andrea; Gotte, Giovanni; Laurents, Douglas

Citation: Fagagnini, Andrea; Garavis, Miguel; Gomez-Pinto, Irene; Fasoli, Sabrina; Gotte, Giovanni; Laurents, Douglas. "NMR Characterization of Angiogenin Variants and tRNA Ala Products Impacting Aberrant Protein Oligomerization"  Int. J. Mol. Sci. 22, 1439-1439 (2021).

Assembly members:
entity_1, polymer, 123 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pAF

Entity Sequences (FASTA):
entity_1: QDNSRYTHFLTQHYDAKPQG RDDRYCESIMRRRGLTSPCK DINTFIHGNKRSIKAICENK NGNPHRENLRISKSSFQVTT CKLHGGSPWPPCQYRATAGF RNVVVACENGLPVHLDQSIF RRP

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts100
1H chemical shifts187

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1human Angiogenin monomer1

Entities:

Entity 1, human Angiogenin monomer 123 residues - Formula weight is not available

1   GLNASPASNSERARGTYRTHRHISPHELEU
2   THRGLNHISTYRASPALALYSPROGLNGLY
3   ARGASPASPARGTYRCYSGLUSERILEMET
4   ARGARGARGGLYLEUTHRSERPROCYSLYS
5   ASPILEASNTHRPHEILEHISGLYASNLYS
6   ARGSERILELYSALAILECYSGLUASNLYS
7   ASNGLYASNPROHISARGGLUASNLEUARG
8   ILESERLYSSERSERPHEGLNVALTHRTHR
9   CYSLYSLEUHISGLYGLYSERPROTRPPRO
10   PROCYSGLNTYRARGALATHRALAGLYPHE
11   ARGASNVALVALVALALACYSGLUASNGLY
12   LEUPROVALHISLEUASPGLNSERILEPHE
13   ARGARGPRO

Samples:

sample_1: human Angiogenin, [U-98% 13C; U-98% 15N], 0.75 ± 0.2 mM; Na2HPO4 / NaH2PO4 buffer 50 mM

sample_conditions_1: ionic strength: 0.10 M; pH: 6.5; pressure: 0.92 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1D 15Nsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1 - collection, processing

SPARKY v3.13 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts