BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50649

Title: 1H, 13C, and 15N backbone chemical shift assignments of yeast sulfhydryl oxidase Erv1   PubMed: 33971209

Deposition date: 2020-12-15 Original release date: 2021-05-21

Authors: Volkov, Alexander; Peker, Esra; Riemer, Jan

Citation: Peker, Esra; Erdogan, Alican; Volkov, Alexander; Riemer, Jan. "Erv1 and cytochrome c mediate rapid electron transfer via a collision-type interaction"  J. Mol. Biol. ., .-. (2021).

Assembly members:
entity_1, polymer, 115 residues, Formula weight is not available
entity_FAD, non-polymer, 785.550 Da.

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a(+)

Entity Sequences (FASTA):
entity_1: MPPDVEQLGRSSWTLLHSVA ASYPAQPTDQQKGEMKQFLN IFSHIYPCNWCAKDFEKYIR ENAPQVESREELGRWMCEAH NKVNKKLRKPKFDCNFWEKR WKDGWDELEHHHHHH

Data sets:
Data typeCount
13C chemical shifts265
15N chemical shifts84
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Erv11
2FAD2

Entities:

Entity 1, Erv1 115 residues - Formula weight is not available

1   METPROPROASPVALGLUGLNLEUGLYARG
2   SERSERTRPTHRLEULEUHISSERVALALA
3   ALASERTYRPROALAGLNPROTHRASPGLN
4   GLNLYSGLYGLUMETLYSGLNPHELEUASN
5   ILEPHESERHISILETYRPROCYSASNTRP
6   CYSALALYSASPPHEGLULYSTYRILEARG
7   GLUASNALAPROGLNVALGLUSERARGGLU
8   GLULEUGLYARGTRPMETCYSGLUALAHIS
9   ASNLYSVALASNLYSLYSLEUARGLYSPRO
10   LYSPHEASPCYSASNPHETRPGLULYSARG
11   TRPLYSASPGLYTRPASPGLULEUGLUHIS
12   HISHISHISHISHIS

Entity 2, FAD - C27 H33 N9 O15 P2 - 785.550 Da.

1   FAD

Samples:

sample_1: Erv1, [U-100% 13C; U-100% 15N; U-95% 2H], 0.7 mM; sodium phosphate 20 mM; sodium chloride 10 mM

sample_conditions_1: ionic strength: 28 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

NMRPipe - processing

CcpNMR - chemical shift assignment, data analysis

qMDD - processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts