BMRB Entry 50642

Title:
1H, 13C, 15N Chemical Shift Assignments for the L75G mutant of Myristoylated HIV-1 Matrix
Deposition date:
2020-12-14
Original release date:
2021-01-26
Authors:
Eastep, Gunnar; Ghanam, Ruba; Green, Todd; Saad, Jamil
Citation:

Citation: Eastep, Gunnar; Ghanam, Ruba; Green, Todd; Saad, Jamil. "Structural characterization of HIV-1 matrix mutants implicated in envelope incorporation"  J. Biol. Chem. 296, 100321-100321 (2021).
PubMed: 33485964

Assembly members:

Assembly members:
entity_1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-19b

Data sets:
Data typeCount
13C chemical shifts127
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1_L75G_myrMA1

Entities:

Entity 1, HIV-1_L75G_myrMA 138 residues - Formula weight is not available

1   MYRGLYALAARGALASERVALLEUSERGLY
2   GLYGLULEUASPLYSTRPGLULYSILEARG
3   LEUARGPROGLYGLYLYSLYSGLNTYRLYS
4   LEULYSHISILEVALTRPALASERARGGLU
5   LEUGLUARGPHEALAVALASNPROGLYLEU
6   LEUGLUTHRSERGLUGLYCYSARGGLNILE
7   LEUGLYGLNLEUGLNPROSERLEUGLNTHR
8   GLYSERGLUGLUGLYARGSERLEUTYRASN
9   THRILEALAVALLEUTYRCYSVALHISGLN
10   ARGILEASPVALLYSASPTHRLYSGLUALA
11   LEUASPLYSILEGLUGLUGLUGLNASNLYS
12   SERLYSLYSLYSALAGLNGLNALAALAALA
13   ASPTHRGLYASNASNSERGLNVALSERGLN
14   ASNTYRHISHISHISHISHISHIS

Samples:

sample_1: HIV-1 L75G myrMA, [U-100% 13C; U-100% 15N], 0.250 mM; sodium phosphate 50 mM; sodium chloride 50 mM; TCEP 1 mM

sample_conditions_1: pH: 6.0; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMRPipe - processing

istHMS - processing

NMRDraw - processing

NMR spectrometers:

  • Bruker AVANCE II 700 MHz
  • Bruker AVANCE III-HD 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks