BMRB Entry 50638

Name: 13C, 15N and 1H assignment of the homeodomain of human SIX1
Published: 2021-12-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50638

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

13C, 15N and 1H assignment of the homeodomain of human SIX1

Deposition date:

2020-12-13

Original release date:

2021-12-14

Authors:

Li, Yan; Ng, Elizabeth; Loh, Ying Ru; Gea, Chong Yu; Huang, Qiwei; Li, Qingxin; Kang, congbao

Citation:

Citation: Li, Yan; Ng, Elizabeth; Loh, Ying Ru; Gea, Chong Yu; Huang, Qiwei; Li, Qingxin; Kang, congbao. "Secondary structures, dynamics, and DNA binding of the homeodomain of human SIX1" J. Pept. Sci. 28, e3376-e3376 (2022).
PubMed: 34713534

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MGEETSYCFKEKSRGVLREW YAHNPYPSPREKRELAEATG LTTTQVSNWFKNRRQRDRAA EAK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	261
15N chemical shifts	62
1H chemical shifts	414

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SIX1 HD	1

Entities:

Entity 1, SIX1 HD 83 residues - Formula weight is not available

MGSSHHHHHHSSGLVPRGSHM is the fusion tag for aiding protein purification

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

GLY

GLU

THR

SER

TYR

CYS

PHE

LYS

4

GLU

LYS

SER

ARG

GLY

VAL

LEU

ARG

GLU

TRP

5

TYR

ALA

HIS

ASN

PRO

TYR

PRO

SER

PRO

ARG

6

GLU

LYS

ARG

GLU

LEU

ALA

GLU

ALA

THR

GLY

7

LEU

THR

GLN

VAL

SER

ASN

TRP

PHE

8

LYS

ASN

ARG

GLN

ARG

ASP

ARG

ALA

9

GLU

ALA

LYS

Samples:

sample_1: SIX1 HD, [U-100% 13C; U-100% 15N], 1 ± 0.1 mM; DTT 1 ± 0.1 mM; D2O 10 ± 1 %; HEPES 20 ± 1 mM; NaCl 150 ± 1 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMRView - data analysis

TOPSPIN v2.1 - collection

NMRPipe - processing

CYANA - processing

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks