BMRB Entry 50630

Title:
Hinge region of the Trypanosoma brucei PKA regulatory subunit
Deposition date:
2020-12-09
Original release date:
2021-01-25
Authors:
Araujo, Nelson; Bruix, Marta; Laurents, Douglas
Citation:

Citation: Araujo, Nelson; Bruix, Marta; Laurents, Douglas. "Disorder and partial folding in the regulatory subunit hinge region of Trypanosoma brucei protein kinase A: The C-linker portion inhibits the parasite's protein kinase A"  Arch. Biochem. Biophys. 698, 108731-108731 (2021).
PubMed: 33359563

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: Synthetic peptides

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGRKRRTTVRGEGIDPEKAK SYVAPY

Data sets:
Data typeCount
13C chemical shifts48
1H chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hinge region1

Entities:

Entity 1, Hinge region 26 residues - Formula weight is not available

1   GLYGLYARGLYSARGARGTHRTHRVALARG
2   GLYGLUGLYILEASPPROGLULYSALALYS
3   SERTYRVALALAPROTYR

Samples:

sample_1: Hinge region of the PKA regulatory subunit 1.9 mM; DSS 0.04 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1 - Data acquisition

SPARKY v3.114 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz