BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 50616

Title: A3T3 DNA   PubMed: 33689312

Deposition date: 2020-12-02 Original release date: 2021-05-28

Authors: Meints, Gary

Citation: Westwood, M; Ljunggren, K; Boyd, Benjamin; Becker, Jaclyn; Dwyer, Tammy; Meints, Gary. "Single-Base Lesions and Mismatches Alter the Backbone Conformational Dynamics in DNA."  Biochemistry 60, 873-885 (2021).

Assembly members:
DNA, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA: CGCAAATTTGCG

Data typeCount
1H chemical shifts78
31P chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA1

Entities:

Entity 1, DNA 12 residues - Formula weight is not available

1   DCDGDCDADADADTDTDTDG
2   DCDG

Samples:

sample_1: DNA 1.0 ± 0.1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 278 K

sample_conditions_2: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 283 K

sample_conditions_3: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 288 K

sample_conditions_4: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 293 K

sample_conditions_5: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

sample_conditions_6: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 303 K

sample_conditions_7: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 308 K

sample_conditions_8: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_5
2D 1H-1H NOESYsample_1isotropicsample_conditions_5
2D 1H-31P HSQCsample_1isotropicsample_conditions_5
1D 31Psample_1isotropicsample_conditions_2
1D 31Psample_1isotropicsample_conditions_3
1D 31Psample_1isotropicsample_conditions_4
1D 31Psample_1isotropicsample_conditions_5
1D 31Psample_1isotropicsample_conditions_6
1D 31Psample_1isotropicsample_conditions_7
1D 31Psample_1isotropicsample_conditions_8

Software:

VNMRj v4.2 - collection

NMR spectrometers:

  • Varian INOVA 400 MHz