BMRB Entry 50612

Name: NMR assignments of ARID4B Tudor domain
Published: 2021-05-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50612

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignments of ARID4B Tudor domain

Deposition date:

2020-12-01

Original release date:

2021-05-28

Authors:

Ren, Jie; Yao, Hongwei; Hu, Wanhui; Feng, Yingang; Perrett, Sarah; Gong, Weibin

Citation:

Citation: Ren, Jie; Yao, Hongwei; Hu, Wanhui; Perrett, Sarah; Gong, Weibin; Feng, Yingang. "Structural basis for the DNA-binding activity of human ARID4B Tudor domain." J. Biol. Chem. 296, 100506-100506 (2021).
PubMed: 33675746

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, 16880 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKALDEPPYLTVGTDVSAKY RGAFCEAKIKTAKRLVKVKV TFRHDSSTVEVQDDHIKGPL KVGAIVEVKNLDGAYQEAVI NKLTDASWYTVVFDDGDEKT LRRSSLCLKGERHFAESETL DQLPLTNPEHFGTPVIGKKT NRGRRSNHIPE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	629
15N chemical shifts	150
1H chemical shifts	995

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ARID4B Tudor domain	1

Entities:

Entity 1, ARID4B Tudor domain 151 residues - 16880 Da.

Tudor domain and C-terminal extended disordered region of ARID4B

1

MET

LYS

ALA

LEU

ASP

GLU

PRO

TYR

LEU

2

THR

VAL

GLY

THR

ASP

VAL

SER

ALA

LYS

TYR

3

ARG

GLY

ALA

PHE

CYS

GLU

ALA

LYS

ILE

LYS

4

THR

ALA

LYS

ARG

LEU

VAL

LYS

VAL

LYS

VAL

5

THR

PHE

ARG

HIS

ASP

SER

THR

VAL

GLU

6

VAL

GLN

ASP

HIS

ILE

LYS

GLY

PRO

LEU

7

LYS

VAL

GLY

ALA

ILE

VAL

GLU

VAL

LYS

ASN

8

LEU

ASP

GLY

ALA

TYR

GLN

GLU

ALA

VAL

ILE

9

ASN

LYS

LEU

THR

ASP

ALA

SER

TRP

TYR

THR

10

VAL

PHE

ASP

GLY

ASP

GLU

LYS

THR

11

LEU

ARG

SER

LEU

CYS

LEU

LYS

GLY

12

GLU

ARG

HIS

PHE

ALA

GLU

SER

GLU

THR

LEU

13

ASP

GLN

LEU

PRO

LEU

THR

ASN

PRO

GLU

HIS

14

PHE

GLY

THR

PRO

VAL

ILE

GLY

LYS

THR

15

ASN

ARG

GLY

ARG

SER

ASN

HIS

ILE

PRO

16

GLU

Samples:

sample_1: ARID4B Tudor domain, [U-99% 13C; U-99% 15N], 0.6 mM; D2O, [U-100% 2H], 10%; DTT 2 mM; EDTA 2 mM; DSS 0.02%; sodium phosphate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.14 M; pH: 7; pressure: 1 atm; temperature: 302 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRViewJ - chemical shift assignment, data analysis

CYANA - structure solution

CNS - refinement

SANE - refinement

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 800 MHz

UNP	Q4LE39
AlphaFold	Q9Y6E1