BMRB Entry 50575

Title:
Cyclosporin E in chloroform
Deposition date:
2020-11-17
Original release date:
2020-11-20
Authors:
Efimov, Sergey; Kobchikova, Polina; Klochkov, Vladimir
Citation:

Citation: Efimov, Sergey; Dubinin, Mikhail; Kobchikova, Polina; Zgadzay, Yuriy; Khodov, Ilya; Belosludtsev, Konstantin; Klochkov, Vladimir. "Comparison of cyclosporin variants B-E based on their structural properties and activity in mitochondrial membranes"  Biochem. Biophys. Res. Commun. 526, 1054-1060 (2020).
PubMed: 32307084

Assembly members:

Assembly members:
entity_1, polymer, 11 residues, 1188.6 Da.

Natural source:

Natural source:   Common Name: Tolypocladium inflatum   Taxonomy ID: 29910   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Tolypocladium inflatum

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XXGLVLAXLLV

Data sets:
Data typeCount
13C chemical shifts61
15N chemical shifts11
1H chemical shifts91
coupling constants19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CsE peptide1

Entities:

Entity 1, CsE peptide 11 residues - 1188.6 Da.

1   BMTABUSARMLEVALMLEALADALMLEMLE
2   VAL

Samples:

sample_1: CsE 2 mM

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

TOPSPIN v3.2 - collection

SPARKY - peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks