Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50528
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Citation: Sanz-Hernandez, Maximo; De Simone, Alfonso. "Backbone NMR assignments of the C-terminal domain of the human prion protein and its disease-associated T183A variant" Biomol. NMR Assign. 15, 193-196 (2021).
PubMed: 33590433
Assembly members:
entity_1, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 TEV
Entity Sequences (FASTA):
entity_1: LGGYMLGSAMSRPIIHFGSD
YEDRYYRENMHRYPNQVYYR
PMDEYSNQNNFVHDCVNIAI
KQHTVTTTTKGENFTETDVK
MMERVVEQMCITQYERESQA
YYQRGS
Data type | Count |
13C chemical shifts | 199 |
15N chemical shifts | 89 |
1H chemical shifts | 89 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | T183A Human Prion Protein (residues 125-230) | 1 |
Entity 1, T183A Human Prion Protein (residues 125-230) 106 residues - Formula weight is not available
1 | LEU | GLY | GLY | TYR | MET | LEU | GLY | SER | ALA | MET | ||||
2 | SER | ARG | PRO | ILE | ILE | HIS | PHE | GLY | SER | ASP | ||||
3 | TYR | GLU | ASP | ARG | TYR | TYR | ARG | GLU | ASN | MET | ||||
4 | HIS | ARG | TYR | PRO | ASN | GLN | VAL | TYR | TYR | ARG | ||||
5 | PRO | MET | ASP | GLU | TYR | SER | ASN | GLN | ASN | ASN | ||||
6 | PHE | VAL | HIS | ASP | CYS | VAL | ASN | ILE | ALA | ILE | ||||
7 | LYS | GLN | HIS | THR | VAL | THR | THR | THR | THR | LYS | ||||
8 | GLY | GLU | ASN | PHE | THR | GLU | THR | ASP | VAL | LYS | ||||
9 | MET | MET | GLU | ARG | VAL | VAL | GLU | GLN | MET | CYS | ||||
10 | ILE | THR | GLN | TYR | GLU | ARG | GLU | SER | GLN | ALA | ||||
11 | TYR | TYR | GLN | ARG | GLY | SER |
sample_1: Human prion protein (residues 125-230), [U-95% 13C; U-95% 15N], 100 uM; sodium phosphate 100 mM
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 289 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
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