BMRB Entry 50528

Title:
Backbone chemical shift assignments for the C-terminal domain of T183A human prion protein (residues 125-230) at 16deg C, pH 7.
Deposition date:
2020-10-22
Original release date:
2021-02-17
Authors:
Sanz-Hernandez, Maximo; De Simone, Alfonso
Citation:

Citation: Sanz-Hernandez, Maximo; De Simone, Alfonso. "Backbone NMR assignments of the C-terminal domain of the human prion protein and its disease-associated T183A variant"  Biomol. NMR Assign. 15, 193-196 (2021).
PubMed: 33590433

Assembly members:

Assembly members:
entity_1, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET His6 TEV

Data sets:
Data typeCount
13C chemical shifts199
15N chemical shifts89
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1T183A Human Prion Protein (residues 125-230)1

Entities:

Entity 1, T183A Human Prion Protein (residues 125-230) 106 residues - Formula weight is not available

1   LEUGLYGLYTYRMETLEUGLYSERALAMET
2   SERARGPROILEILEHISPHEGLYSERASP
3   TYRGLUASPARGTYRTYRARGGLUASNMET
4   HISARGTYRPROASNGLNVALTYRTYRARG
5   PROMETASPGLUTYRSERASNGLNASNASN
6   PHEVALHISASPCYSVALASNILEALAILE
7   LYSGLNHISTHRVALTHRTHRTHRTHRLYS
8   GLYGLUASNPHETHRGLUTHRASPVALLYS
9   METMETGLUARGVALVALGLUGLNMETCYS
10   ILETHRGLNTYRGLUARGGLUSERGLNALA
11   TYRTYRGLNARGGLYSER

Samples:

sample_1: Human prion protein (residues 125-230), [U-95% 13C; U-95% 15N], 100 uM; sodium phosphate 100 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 289 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe - data analysis

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Related Database Links:

UNP P04156
AlphaFold Q9UP19

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks