BMRB Entry 50527

Name: Backbone chemical shift assignments for the C-terminal domain of wild type human prion protein (residues 125-230) at 16deg C, pH 7.
Published: 2021-02-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50527

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments for the C-terminal domain of wild type human prion protein (residues 125-230) at 16deg C, pH 7.

Deposition date:

2020-10-22

Original release date:

2021-02-17

Authors:

Sanz-Hernandez, Maximo; De Simone, Alfonso

Citation:

Citation: Sanz-Hernandez, Maximo; De Simone, Alfonso. "Backbone NMR assignments of the C-terminal domain of the human prion protein and its disease-associated T183A variant" Biomol. NMR Assign. 15, 193-196 (2021).
PubMed: 33590433

Assembly members:

Assembly members:
entity_1, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LGGYMLGSAMSRPIIHFGSD YEDRYYRENMHRYPNQVYYR PMDEYSNQNNFVHDCVNITI KQHTVTTTTKGENFTETDVK MMERVVEQMCITQYERESQA YYQRGS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	274
15N chemical shifts	95
1H chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human Prion Protein (residues 125-230)	1

Entities:

Entity 1, Human Prion Protein (residues 125-230) 106 residues - Formula weight is not available

1

LEU

GLY

TYR

MET

LEU

GLY

SER

ALA

MET

2

SER

ARG

PRO

ILE

HIS

PHE

GLY

SER

ASP

3

TYR

GLU

ASP

ARG

TYR

ARG

GLU

ASN

MET

4

HIS

ARG

TYR

PRO

ASN

GLN

VAL

TYR

ARG

5

PRO

MET

ASP

GLU

TYR

SER

ASN

GLN

ASN

6

PHE

VAL

HIS

ASP

CYS

VAL

ASN

ILE

THR

ILE

7

LYS

GLN

HIS

THR

VAL

THR

LYS

8

GLY

GLU

ASN

PHE

THR

GLU

THR

ASP

VAL

LYS

9

MET

GLU

ARG

VAL

GLU

GLN

MET

CYS

10

ILE

THR

GLN

TYR

GLU

ARG

GLU

SER

GLN

ALA

11

TYR

GLN

ARG

GLY

SER

Samples:

sample_1: Human prion protein (residues 125-230), [U-95% 13C; U-95% 15N], 100 uM; sodium phosphate 100 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 289 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

NMR spectrometers:

Bruker AVANCE III 800 MHz

UNP	P04156
AlphaFold	Q9UP19