BMRB Entry 50524

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for c-FLIPs_C8-1a compex
Deposition date:
2020-10-20
Original release date:
2022-10-14
Authors:
Hu, Kaifeng; Bai, Zhiqiang
Citation:

Citation: Hu, Kaifeng; Bai, Zhiqiang. "Expression, purification, and characterization of c-FLIP tandem death effector domains from Escherichia coli"  Protein Expr. Purif. 200, 106168-106168 (2022).
PubMed: 36084903

Assembly members:

Assembly members:
entity_1, polymer, 185 residues, Formula weight is not available
entity_2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Data sets:
Data typeCount
13C chemical shifts746
15N chemical shifts179
1H chemical shifts1259

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1c-FLIPs1
2C8-1a2

Entities:

Entity 1, c-FLIPs 185 residues - Formula weight is not available

1   METGLYARGSERASPSERALAGLUVALILE
2   HISGLNVALGLUGLUALALEUASPTHRASP
3   GLULYSGLUMETLEULEUPHELEUCYSARG
4   ASPVALALAILEASPVALVALPROPROASN
5   VALARGASPLEULEUASPILELEUARGGLU
6   ARGGLYLYSLEUSERVALGLYASPLEUALA
7   GLULEULEUTYRARGVALARGARGPHEASP
8   LEULEULYSARGILELEULYSMETASPARG
9   LYSALAVALGLUTHRHISLEULEUARGASN
10   PROHISLEUVALSERASPTYRARGVALLEU
11   METALAGLUILEGLYGLUASPLEUASPLYS
12   SERASPVALSERSERLEUILEGLYLEUMET
13   LYSASPTYRMETGLYARGGLYLYSILESER
14   LYSGLULYSSERPHELEUASPLEUVALVAL
15   GLULEUGLULYSLEUASNLEUVALALAPRO
16   ASPGLNLEUASPLEULEUGLULYSCYSLEU
17   LYSASNILEHISARGILEASPLEULYSTHR
18   LYSILEGLNLYSTYRLYSGLNLEUGLUHIS
19   HISHISHISHISHIS

Entity 2, C8-1a 8 residues - Formula weight is not available

1   ASPPHESERARGASNLEUTYRASP

Samples:

sample_1: c-FLIPs, [U-99% 13C; U-99% 15N], 0.6 mM; C8-1a 4.2 mM

sample_2: C8-1a 10 mM; c-FLIPs, [U-100% 15N], 0.6 mM

sample_conditions_1: ionic strength: 110 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 110 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 13C-15N Filtered NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-1H ROESYsample_2isotropicsample_conditions_2
2D 1H-1H 13C-15N Filtered NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks