BMRB Entry 50523

Name: SsoSSB 1-114
Published: 2021-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50523

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SsoSSB 1-114

Deposition date:

2020-10-20

Original release date:

2021-06-30

Authors:

Yang, Min June; Park, Chin-Ju; Lee, Woonghee

Citation:

Citation: Yang, Min June; Lee, Woonghee; Park, Chin-Ju. "Resonance assignments and secondary structure of thermophile single-stranded DNA binding protein from Sulfolobus solfataricus at 323K." Biomol. NMR Assign. 15, 159-164 (2021).
PubMed: 33405014

Assembly members:

Assembly members:
entity_1, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Saccharolobus solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEEKVGNLKPNMESVNVTVR VLEASEARQIQTKNGVRTIS EAIVGDETGRVKLTLWGKHA GSIKEGQVVKIENAWTTAFK GQVQLNAGSKTKIAEASEDG FPESSQIPENTPTAENLYFQ SGSHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	483
15N chemical shifts	116
1H chemical shifts	756

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SsoSSB	1

Entities:

Entity 1, SsoSSB 129 residues - Formula weight is not available

1

MET

GLU

LYS

VAL

GLY

ASN

LEU

LYS

PRO

2

ASN

MET

GLU

SER

VAL

ASN

VAL

THR

VAL

ARG

3

VAL

LEU

GLU

ALA

SER

GLU

ALA

ARG

GLN

ILE

4

GLN

THR

LYS

ASN

GLY

VAL

ARG

THR

ILE

SER

5

GLU

ALA

ILE

VAL

GLY

ASP

GLU

THR

GLY

ARG

6

VAL

LYS

LEU

THR

LEU

TRP

GLY

LYS

HIS

ALA

7

GLY

SER

ILE

LYS

GLU

GLY

GLN

VAL

LYS

8

ILE

GLU

ASN

ALA

TRP

THR

ALA

PHE

LYS

9

GLY

GLN

VAL

GLN

LEU

ASN

ALA

GLY

SER

LYS

10

THR

LYS

ILE

ALA

GLU

ALA

SER

GLU

ASP

GLY

11

PHE

PRO

GLU

SER

GLN

ILE

PRO

GLU

ASN

12

THR

PRO

THR

ALA

GLU

ASN

LEU

TYR

PHE

GLN

13

SER

GLY

SER

HIS

Samples:

sample_1: SsoSSB, [U-100% 13C; U-100% 15N], 1.2 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 323 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - data analysis

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks