BMRB Entry 50517

Title:
Nsp3c SUD-C
Deposition date:
2020-10-19
Original release date:
2021-01-25
Authors:
Gallo, Angelo; Tsika, Aikaterini; Fourkiotis, Nikolaos; Spyroulias, Georgios
Citation:

Citation: Gallo, Angelo; Tsika, Aikaterini; Fourkiotis, Nikolaos; Cantini, Francesca; Banci, Lucia; Sreeramulu, Sridhar; Schwalbe, Harald; Spyroulias, Georgios. "1H,13C and 15N chemical shift assignments of the SUD domains of SARS-CoV-2 non-structural protein 3c: The SUD-M and SUD-C domains"  Biomol. NMR Assign. 15, 165-171 (2021).
PubMed: 33423172

Assembly members:

Assembly members:
entity_1, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex4T-1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts67
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nsp3c_SUD_C1

Entities:

Entity 1, Nsp3c_SUD_C 66 residues - Formula weight is not available

1   GLYSERGLUGLUHISPHEILEGLUTHRILE
2   SERLEUALAGLYSERTYRLYSASPTRPSER
3   TYRSERGLYGLNSERTHRGLNLEUGLYILE
4   GLUPHELEULYSARGGLYASPLYSSERVAL
5   TYRTYRTHRSERASNPROTHRTHRPHEHIS
6   LEUASPGLYGLUVALILETHRPHEASPASN
7   LEULYSTHRLEULEUSER

Samples:

sample_1: entity_1, [U-13C; U-15N], 0.73 mM; NaPi 50 mM; NaCl 50 mM; DTT 2 mM; EDTA 2 mM

sample_conditions_1: ionic strength: 104 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
(Hb)CB(CgCd)HDsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D HSQC HCsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
(Hb)CB(CgCdCe)HEsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
HBHA(CBCACO)NHsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
(H)CCH-TOCSY 3Dsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1

Software:

LOGS v2.2 - collection

CARA - chemical shift assignment, peak picking

TOPSPIN v4.0.6 - collection

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks